Anti-Villin antibody (ab97512)
Key features and details
- Rabbit polyclonal to Villin
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Villin antibody
See all Villin primary antibodies -
Description
Rabbit polyclonal to Villin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 7-256 of Human Villin (NP_009058).
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Positive control
- WB: HepG2 cell lysate, mouse and rat kidney extract. IHC-P: A549 xenograft tissue, mouse duodenum and kidney, rat colon, kidney. IF: HeLa whole cell.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF | (1) |
1/100 - 1/1000.
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WB |
1/500 - 1/10000. Predicted molecular weight: 93 kDa.
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IHC-P |
1/100 - 1/1000.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/10000. Predicted molecular weight: 93 kDa. |
IHC-P
1/100 - 1/1000. |
Target
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Function
Ca(2+)-regulated actin-binding protein. -
Tissue specificity
Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. -
Sequence similarities
Belongs to the villin/gelsolin family.
Contains 6 gelsolin-like repeats.
Contains 1 HP (headpiece) domain. -
Domain
Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 7429 Human
- Entrez Gene: 22349 Mouse
- Entrez Gene: 316521 Rat
- Omim: 193040 Human
- SwissProt: P09327 Human
- SwissProt: Q62468 Mouse
- Unigene: 654595 Human
- Unigene: 471601 Mouse
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Alternative names
- D2S1471 antibody
- OTTHUMP00000164145 antibody
- VIL antibody
see all
Images
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Anti-Villin antibody (ab97512) at 1/2000 dilution + HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 93 kDa7.5% SDS-PAGE
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Paraffin-embedded A549 xenograft tissue stained Villin using ab97512 at a 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Confocal immunofluorescence analysis of methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling Villin with ab97512 (green) at 1/500 dilution. Red: Alpha-tubulin filaments, alpha Tubulin antibody at 1/2000.
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Anti-Villin antibody (ab97512) at 1/1000 dilution + Mouse kidney tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 93 kDa7.5% SDS-PAGE
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Paraffin-embedded mouse duodenum tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
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Anti-Villin antibody (ab97512) at 1/2000 dilution + Rat kidney tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 93 kDa7.5% SDS-PAGE
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Paraffin-embedded mouse duodenum tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
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Paraffin-embedded rat kidney tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
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Paraffin-embedded mouse kidney tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
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Paraffin-embedded rat colon tissue stained Villin at cytoplasm using ab97512 at 1/500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15min.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab97512 has been referenced in 4 publications.
- Wen H et al. Ruscogenins Improve CD-Like Enteritis by Inhibiting Apoptosis of Intestinal Epithelial Cells and Activating Nrf2/NQO1 Pathway. Oxid Med Cell Longev 2022:4877275 (2022). PubMed: 35308175
- Marrella A et al. In vitro demonstration of intestinal absorption mechanisms of different sugars using 3D organotypic tissues in a fluidic device. ALTEX 37:255-264 (2020). PubMed: 31893489
- Ayehunie S et al. Human Primary Cell-Based Organotypic Microtissues for Modeling Small Intestinal Drug Absorption. Pharm Res 35:72 (2018). PubMed: 29476278
- Bäder S et al. Effect of the actin- and calcium-regulating activities of ITPKB on the metastatic potential of lung cancer cells. Biochem J 475:2057-2071 (2018). PubMed: 29871874