Overview

  • Product name
  • Description
    Rabbit polyclonal to Villin
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 7-256 of Human Villin (NP_009058).

  • Positive control
    • HepG2 whole cell lysate, A549 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab97512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 93 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function
    Ca(2+)-regulated actin-binding protein.
  • Tissue specificity
    Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
  • Sequence similarities
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
    Contains 1 HP (headpiece) domain.
  • Domain
    Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • D2S1471 antibody
    • OTTHUMP00000164145 antibody
    • VIL antibody
    • VIL1 antibody
    • VILI_HUMAN antibody
    • Villin 1 antibody
    • Villin-1 antibody
    • Villin1 antibody
    see all

Images

  • Anti-Villin antibody (ab97512) at 1/5000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 93 kDa
  • Immunohistochemical analysis of Villin in paraffin embedded A549 xenograft, using ab97512 at a 1/500 dilution.

References

ab97512 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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