Anti-Villin antibody [SPM226], prediluted (ab54194)

Overview

  • Product nameAnti-Villin antibody [SPM226], prediluted
    See all Villin primary antibodies
  • Description
    Mouse monoclonal [SPM226] to Villin, prediluted
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein (Human)

  • Positive control
    • Small intestine and kidney

Properties

Applications

Our Abpromise guarantee covers the use of ab54194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: Ready to use for 30 min at room temperature.

    Staining of formalin fixed tissue requires boiling tissue sections in 1mM EDTA pH 8.0 for 10-20 min followed by cooling at room temperature for 20 min.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCa(2+)-regulated actin-binding protein.
    • Tissue specificityMajor component of microvilli of intestinal epithelial cells and kidney proximal tubule cells.
    • Sequence similaritiesBelongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
      Contains 1 HP (headpiece) domain.
    • DomainConsists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • D2S1471 antibody
      • OTTHUMP00000164145 antibody
      • VIL antibody
      • VIL1 antibody
      • VILI_HUMAN antibody
      • Villin 1 antibody
      • Villin-1 antibody
      • Villin1 antibody
      see all

    Anti-Villin antibody [SPM226], prediluted images

    • Staining of Villin in Human kidney tissue by immunohistochemistry using ab54194.

    References for Anti-Villin antibody [SPM226], prediluted (ab54194)

    ab54194 has not yet been referenced specifically in any publications.

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