Anti-Vimentin antibody (ab11256)
Key features and details
- Goat polyclonal to Vimentin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Vimentin antibody
See all Vimentin primary antibodies -
Description
Goat polyclonal to Vimentin -
Host species
Goat -
Specificity
No reactivity was observed with the other families of intermediate filaments, including, desmin, keratin, neurofilaments and glial filaments.The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: a wide range of other species -
Immunogen
Tissue, cells or virus corresponding to Human Vimentin. Vimentin from cultured human foreskin fibroblasts.
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General notes
If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.097% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Purification notes
The antiserum has been treated to remove lipoproteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
1/20.
|
Notes |
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IHC-P
1/20. |
Target
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Function
Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. -
Tissue specificity
Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. -
Involvement in disease
Cataract 30 -
Sequence similarities
Belongs to the intermediate filament family. -
Domain
The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. -
Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 7431 Human
- Omim: 193060 Human
- SwissProt: P08670 Human
- Unigene: 455493 Human
- Unigene: 691131 Human
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Form
Vimentin is found in connective tissue and in the cytoskeleton. -
Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab11256 has been referenced in 31 publications.
- Zhang F et al. Identification of a Feed-Forward Loop Between 15(S)-HETE and PGE2 in Human Amnion at Parturition. J Lipid Res 63:100294 (2022). PubMed: 36206855
- Tian Y et al. Immunosuppressants Tacrolimus and Sirolimus revert the cardiac antifibrotic properties of p38-MAPK inhibition in 3D-multicellular human iPSC-heart organoids. Front Cell Dev Biol 10:1001453 (2022). PubMed: 36438566
- Lewis-Israeli YR et al. Generating Self-Assembling Human Heart Organoids Derived from Pluripotent Stem Cells. J Vis Exp N/A:N/A (2021). PubMed: 34605811
- Fan X et al. Hsa_circRNA_0045861 promotes renal injury in ureteropelvic junction obstruction via the microRNA-181d-5p/sirtuin 1 signaling axis. Ann Transl Med 9:1571 (2021). PubMed: 34790777
- Ding L et al. Interleukin-1ß Suppresses Gastrin via Primary Cilia and Induces Antral Hyperplasia. Cell Mol Gastroenterol Hepatol 11:1251-1266 (2021). PubMed: 33347972