Other Immunogen Type corresponding to Vimentin. vimentin from cultured human foreskin fibroblasts.
Our Abpromise guarantee covers the use of ab11256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/20. Localizes vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes, as well as tumors derived from these cells including sarcomas, lymphomas, melanomas, and their metastatic lesions.|
|WB||1/50. Detects a band of approximately 58 kDa.
The antibody specifically stains the vimentin band using total cell or tissue extracts.
|ICC||Use at an assay dependent concentration. On normal and pathological tissue of mesenchymal derivation.|
Immunocytochemistry/ Immunofluorescence analysis of mouse sertoli cells labeling Vimentin with ab11256 at 1/20 dilution. Cells were fixed with methanol and blocked with 5% serum for 20 minutes at 25 °C. A polyclonal Donkey anti-goat Dylight® 488 was used as the secondary antibody at 1/400 dilution.