Overview

  • Product name
  • Description
    Goat polyclonal to Vimentin
  • Specificity
    No reactivity was observed with the other families of intermediate filaments, including, desmin, keratin, neurofilaments and glial filaments.The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, WB, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: a wide range of other species
  • Immunogen

    Other Immunogen Type corresponding to Vimentin. vimentin from cultured human foreskin fibroblasts.

  • Positive control
  • General notes
    If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. This goat antiserum was maintained at pH 5.0 for 40 minutes to meet U.S.D.A. requirements.

Properties

Applications

Our Abpromise guarantee covers the use of ab11256 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-P 1/20. Localizes vimentin in fibroblasts, endothelial cells, lymphoid tissues and melanocytes, as well as tumors derived from these cells including sarcomas, lymphomas, melanomas, and their metastatic lesions.
IHC-Fr 1/20.
WB 1/50. Detects a band of approximately 58 kDa.

The antibody specifically stains the vimentin band using total cell or tissue extracts.

ICC Use at an assay dependent concentration. On normal and pathological tissue of mesenchymal derivation.

Target

  • Function
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity
    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease
    Cataract 30
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Domain
    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Form
    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Images

  • ii Panels i-iii, TGF-ß1 induces EMT-type changes in primary hepatocytes. Immunofluorescence staining for E-cadherin (green) and vimentin (red) in primary hepatocytes were evaluated by confocal laser microscopy. DAPI stained nuclei blue. There was apparent near loss of cell membrane-bound E-cadherin in TGF-ß1-treated cells in a time-dependent manner. Diffuse vimentin staining was observed in control cells, and TGF-ß1-treated hepatocytes exhibited a fibrillar vimentin pattern most noted at 48 and 72 h. Also note that TGF-ß1 caused hepatocytes to assume a spindle-shaped morphology, which was not observed in controls. Scale bar, 50 µm.
  • Immunocytochemistry/ Immunofluorescence analysis of mouse sertoli cells labeling Vimentin with ab11256 at 1/20 dilution. Cells were fixed with methanol and blocked with 5% serum for 20 minutes at 25 °C. A polyclonal Donkey anti-goat Dylight® 488 was used as the secondary antibody at 1/400 dilution.

    See Abreview

References

This product has been referenced in:
  • Nagy BM  et al. Lack of ABCG2 Leads to Biventricular Dysfunction and Remodeling in Response to Hypoxia. Front Physiol 8:98 (2017). ICC/IF ; Mouse . Read more (PubMed: 28270772) »
  • Chorghade S  et al. Poly(A) tail length regulates PABPC1 expression to tune translation in the heart. Elife 6:N/A (2017). WB, IF ; Mouse . Read more (PubMed: 28653618) »

See all 15 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Sample
Human Cell (breast cancer cell lines)
Specification
breast cancer cell lines
Permeabilization
Yes - 0.3% Triton X-100 in TBS
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 18 2014

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Breast cancer cell lines)
Specification
Breast cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Dec 10 2014

Yes this antibody has been tested on human paraffin embedded tissue followed by HIER.

The following publication used our antibody in this application on human placenta:

Lockwood CJ et al. Progestin and thrombin regulate tissue factor ...

Read More

The antigen retrieval was performed using 0.1% Trypsin Enzyme incubation for 15 - 25 min. The enzyme was incubated at a temperature of 37°C

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (sertoli cells)
Specification
sertoli cells
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Elad Fadlon

Verified customer

Submitted Aug 06 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Heart)
Specification
Heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Feb 21 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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