Overview

  • Product nameAnti-Vimentin antibody
    See all Vimentin primary antibodies
  • Description
    Chicken polyclonal to Vimentin
  • Tested applicationsSuitable for: ICC/IF, IHC-FoFr, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Vimentin.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab24525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-FoFr 1/5000.
IHC-Fr Use at an assay dependent concentration. PubMed: 22919071
WB 1/10000.

Target

  • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in diseaseCataract 30
  • Sequence similaritiesBelongs to the intermediate filament family.
  • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • FormVimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody images

  • Immunohistochemistry (Frozen sections) analysis of mouse heart tissue sections labelling Vimentin with ab24525. Tissue was fixed in 4% paraformaldehyde in PBS at 4°C overnight, and cyroprotected with 30% sucrose in PBS. The samples were embedded in optimal cutting temperature (OCT) compound, snap-frozen in liquid nitrogen, and stored at −80°C. Paraformaldehyde-fixed cryosections (10 μm) were labeled with the primary antibodies Anti-Vimentin (ab24525) and Anti-GFP (ab6662) and secondary Alexa Fluor-conjugated antibodies. Nuclei were labeled with 0.2 μg/ml DAPI.

  • Immunocytochemistry/ Immunofluorescence analysis of neuron/glial cultures labeling Vimentin with ab24525 (green) and GFAP with ab7260 (red). Vimentin is the sole cytoplasmic intermediate filament subunit expressed in fibroblasts, microglial and endothelial cells. The flattened cells in the middle of the image which appear green are fibroblasts. Astrocytes may express primarily GFAP, or both GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.

  • Rat cerebral cortex cultures stained with chicken antibody to vimentin ab24525 (green) and rabbit antibody to GFAP (red). Note flattened fibroblastic cells are mostly green (i.e. vimentin positive, GFAP negative), while clearly astrocytic cells, express both vimentin and GFAP and therefore appear golden or orange. Certain other cells express predominantly GFAP and therefore appear red.

     

  • Immunohistochemistry (Frozen sections) analysis of mouse dorsal skin wound labelling Vimentin with ab24525 (red) at 1/200 dilution. Dissected wounds were fixed for 2 hrs in 4% PFA and infused with 10% sucrose before cryo-embedding. For cryo-mounting, sucrose-infused wounds were mounted in optimal cutting medium (O.C.T) and frozen in a bath of dry ice and ethanol. 7 µm sections were subsequently cut on a cryo-stat for immuno-histochemistry studies. Cryo-sections were permeabilized in 0.5% Triton-x-100 before blocking with 10% normal goat serum. DAPI nuclear staining.

  • Western blot of Rat whole brain extract,HeLa,SH-SY5Y,HEK293, and NIH/3T3 cells probed with ab24525,showing a single strong band at ~ 50kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue sections labeling Vimentin with ab24525 (undiluted). Cells were fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100. Cells were blocked with 5% normal donkey serum for 1 hour at 22°C, followed by incubation with ab24525 in 5% donkey serum 0.3%tritonx-100 in kpbs for 18 hours at 4°C. A polyclonal donkey anti-chicken AMCA conjugated secondary antibody was used at 1/500 dilution.

    See Abreview

  • Immunohistochemical analysis of murine eye tissue, staining Vimentin (red) with ab24525.

    Tissue was fixed in paraformaldehyde, blocked for 1 h at room temperature in 0.5% BSA, 0.2% Tween-20 plus 5% goat serum. Samples were incubated with primary antibody (1/200) for 1 hour at room temperature. A Cy3-conjugated goat anti-chicken IgG (1/200) was used as the secondary antibody.

  • ab24525 staining Vimentin in rat smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Abcam`s ab6875, goat anti-chicken IgY Texas Red was used as secondary antibody at 1/400 dilution.

    See Abreview

References for Anti-Vimentin antibody (ab24525)

This product has been referenced in:
  • Telegina DV  et al. Contributions of age-related alterations of the retinal pigment epithelium and of glia to the AMD-like pathology in OXYS rats. Sci Rep 7:41533 (2017). ICC/IF ; Rat . Read more (PubMed: 28134357) »
  • Moeendarbary E  et al. The soft mechanical signature of glial scars in the central nervous system. Nat Commun 8:14787 (2017). ICC/IF ; Rat . Read more (PubMed: 28317912) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (E17.5 Embryo Heart Sections - 10um thick)
Permeabilization Yes - 0.5% Tween-20
Specification E17.5 Embryo Heart Sections - 10um thick
Blocking step 5% Secondary specific serum + 1% BSA + 0.5% Tween-20 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted May 17 2017

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step None
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - 0.3% Triton X-100
Fixative Paraformaldehyde
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Submitted Dec 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step goat serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Mouse Tissue sections (skin)
Specification skin
Permeabilization No
Fixative Acetone
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Submitted Aug 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Heart)
Specification Heart
Fixative Methanol
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted May 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Cardiac fibroblasts)
Loading amount 4 µg
Specification Cardiac fibroblasts
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Feb 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (smooth muscle cells from mesenteric artery)
Specification smooth muscle cells from mesenteric artery
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
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Submitted Nov 17 2009

Thank you for your enquiry. Thank you very much for bringing this to our attention. I apologize for the confusion. I'm not sure how both images were added to both datasheets, but I have updated the datasheets with the image that used that particul...

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