Overview

  • Product name
  • Description
    Chicken polyclonal to Vimentin
  • Tested applications
    Suitable for: ICC/IF, IHC-FoFr, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Vimentin.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab24525 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-FoFr 1/5000.
IHC-Fr Use at an assay dependent concentration. PubMed: 22919071
WB 1/10000.

Target

  • Function
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity
    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease
    Cataract 30
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Domain
    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Form
    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody images

  • Immunohistochemistry (Frozen sections) analysis of mouse heart tissue sections labelling Vimentin with ab24525. Tissue was fixed in 4% paraformaldehyde in PBS at 4°C overnight, and cyroprotected with 30% sucrose in PBS. The samples were embedded in optimal cutting temperature (OCT) compound, snap-frozen in liquid nitrogen, and stored at −80°C. Paraformaldehyde-fixed cryosections (10 μm) were labeled with the primary antibodies Anti-Vimentin (ab24525) and Anti-GFP (ab6662) and secondary Alexa Fluor-conjugated antibodies. Nuclei were labeled with 0.2 μg/ml DAPI.

  • Immunocytochemistry/ Immunofluorescence analysis of neuron/glial cultures labeling Vimentin with ab24525 (green) and GFAP with ab7260 (red). Vimentin is the sole cytoplasmic intermediate filament subunit expressed in fibroblasts, microglial and endothelial cells. The flattened cells in the middle of the image which appear green are fibroblasts. Astrocytes may express primarily GFAP, or both GFAP and vimentin, and so appear red (GFAP only) or golden yellow (GFAP and Vimentin). In cells which express both GFAP and vimentin, the two proteins assemble to produce heteropolymer filaments.

  • Rat cerebral cortex cultures stained with chicken antibody to vimentin ab24525 (green) and rabbit antibody to GFAP (red). Note flattened fibroblastic cells are mostly green (i.e. vimentin positive, GFAP negative), while clearly astrocytic cells, express both vimentin and GFAP and therefore appear golden or orange. Certain other cells express predominantly GFAP and therefore appear red.

     

  • Immunohistochemistry (Frozen sections) analysis of mouse dorsal skin wound labelling Vimentin with ab24525 (red) at 1/200 dilution. Dissected wounds were fixed for 2 hrs in 4% PFA and infused with 10% sucrose before cryo-embedding. For cryo-mounting, sucrose-infused wounds were mounted in optimal cutting medium (O.C.T) and frozen in a bath of dry ice and ethanol. 7 µm sections were subsequently cut on a cryo-stat for immuno-histochemistry studies. Cryo-sections were permeabilized in 0.5% Triton-x-100 before blocking with 10% normal goat serum. DAPI nuclear staining.

  • Western blot of Rat whole brain extract,HeLa,SH-SY5Y,HEK293, and NIH/3T3 cells probed with ab24525,showing a single strong band at ~ 50kDa.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat brain tissue sections labeling Vimentin with ab24525 (undiluted). Cells were fixed with paraformaldehyde and permeabilized with 0.3% Triton X-100. Cells were blocked with 5% normal donkey serum for 1 hour at 22°C, followed by incubation with ab24525 in 5% donkey serum 0.3%tritonx-100 in kpbs for 18 hours at 4°C. A polyclonal donkey anti-chicken AMCA conjugated secondary antibody was used at 1/500 dilution.

    See Abreview

  • Immunohistochemical analysis of murine eye tissue, staining Vimentin (red) with ab24525.

    Tissue was fixed in paraformaldehyde, blocked for 1 h at room temperature in 0.5% BSA, 0.2% Tween-20 plus 5% goat serum. Samples were incubated with primary antibody (1/200) for 1 hour at room temperature. A Cy3-conjugated goat anti-chicken IgG (1/200) was used as the secondary antibody.

  • ab24525 staining Vimentin in rat smooth muscle cells from mesenteric artery by Immunocytochemistry/ Immunofluorescence. Cells were fixed with 4% paraformaldehyde in physiological saline solution (PSS) 4 min at 4°C and permeabilized with 0.3% Triton x100 before blocking with 2% BSA was done for 30 minutes at 20°C. Samples were incubated with primary antibody (1/300: in PSS with 2%BSA and 0.3% Triton X-100) for 14 hours at 4°C. An Abcam`s ab6875, goat anti-chicken IgY Texas Red was used as secondary antibody at 1/400 dilution.

    See Abreview

References for Anti-Vimentin antibody (ab24525)

This product has been referenced in:
  • Telegina DV  et al. Contributions of age-related alterations of the retinal pigment epithelium and of glia to the AMD-like pathology in OXYS rats. Sci Rep 7:41533 (2017). ICC/IF ; Rat . Read more (PubMed: 28134357) »
  • Moeendarbary E  et al. The soft mechanical signature of glial scars in the central nervous system. Nat Commun 8:14787 (2017). ICC/IF ; Rat . Read more (PubMed: 28317912) »

See all 13 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E17.5 Embryo Heart Sections - 10um thick)
Permeabilization
Yes - 0.5% Tween-20
Specification
E17.5 Embryo Heart Sections - 10um thick
Blocking step
5% Secondary specific serum + 1% BSA + 0.5% Tween-20 as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 17 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Normal donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Antigen retrieval step
None
Sample
Rat Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
goat serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Tissue sections (skin)
Specification
skin
Permeabilization
No
Fixative
Acetone
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Verified customer

Submitted Aug 28 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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Submitted May 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Cardiac fibroblasts)
Loading amount
4 µg
Specification
Cardiac fibroblasts
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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Submitted Feb 04 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (smooth muscle cells from mesenteric artery)
Specification
smooth muscle cells from mesenteric artery
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 17 2009

Thank you for your enquiry. Thank you very much for bringing this to our attention. I apologize for the confusion. I'm not sure how both images were added to both datasheets, but I have updated the datasheets with the image that used that particul...

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