Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab39376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/200.
IHC-Fr 1/100 - 1/200.
ICC/IF 1/100 - 1/200.
WB 1/250 - 1/500. Predicted molecular weight: 54 kDa.

Target

  • Function
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity
    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease
    Cataract 30
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Domain
    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Form
    Vimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody images

  • Cultured dissociated cells from an e13 mouse brain), vimentin (green staining) can be seen forming the cytoskeleton of immature astrocyte progenitor cells. DAPI (blue staining) allows visualization of nuclei.
  • ab39376 at a 1/200 dilution staining Vimentin in mouse skin tissue by Immunohistochemistry (Frozen sections).
    Tissue was flash frozen in liquid nitrogen. Skin fibroblasts, but not hematopoietic cells, are infected in WT mice. Immunofluorescence was performed 1 day after ID infection with WT CHIKV at the site of inoculation into the ear. Cell nuclei were stained with DAPI.

References for Anti-Vimentin antibody (ab39376)

This product has been referenced in:
  • Schilte C  et al. Type I IFN controls chikungunya virus via its action on nonhematopoietic cells. J Exp Med 207:429-42 (2010). IHC-Fr . Read more (PubMed: 20123960) »
  • Lawson DA  et al. Basal epithelial stem cells are efficient targets for prostate cancer initiation. Proc Natl Acad Sci U S A 107:2610-5 (2010). ICC/IF ; Mouse . Read more (PubMed: 20133806) »

See all 2 Publications for this product

Product Wall

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The area on the antibody datasheet that is labeled "Cross-reactivity" is a list of species that the antibody reacts with. For ab393676, anti-vimentin, the two species that the antibody is known to react with are ...

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Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cornea epithelial Cells)
Specification
Cornea epithelial Cells
Fixative
Methanol
Permeabilization
Yes - 0.2% Triton-X 15mins
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Username

Miss. Ashley Quintana

Verified customer

Submitted Aug 17 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Monkey Tissue sections (cerebellum)
Specification
cerebellum
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 13 2010

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (kidney)
Specification
kidney
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 27 2008

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Skin)
Specification
Skin
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Precedia Massey

Verified customer

Submitted Jan 11 2008

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mammary tumor)
Specification
mammary tumor
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Aug 28 2007

Thank you for your patience. I am sorry to hear that you are experiencing difficulties with this product ab11256 and ab39376 in immunofluorescence. Often it is possible to make suggestions that help resolve problems. We will happily offer technical...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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