Overview

  • Product nameAnti-Vimentin antibody
    See all Vimentin primary antibodies
  • Description
    Rabbit polyclonal to Vimentin
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant human protein purified from E.coli (His-Vimentin)

  • Positive control
    • RD2, C6, HeLa and NIH3T3 cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab71144 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
IP Use at an assay dependent concentration. Use 4 µl for 200 µg lysate.

Target

  • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in diseaseCataract 30
  • Sequence similaritiesBelongs to the intermediate filament family.
  • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • FormVimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody images

  • All lanes : Anti-Vimentin antibody (ab71144) at 1/5000 dilution

    Lane 1 : RD2 cell lysate
    Lane 2 : C6 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : NIH3T3 cell lysate


    Predicted band size : 54 kDa
    Observed band size : 54 kDa

References for Anti-Vimentin antibody (ab71144)

This product has been referenced in:
  • Lang BJ  et al. Heat stress induces epithelial plasticity and cell migration independent of heat shock factor 1. Cell Stress Chaperones : (2012). Read more (PubMed: 22791010) »
  • De Ceuleneer M  et al. Quantification of citrullination by means of skewed isotope distribution pattern. J Proteome Res 11:5245-51 (2012). Read more (PubMed: 22978259) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela)
Loading amount 100 µg
Specification Hela
Gel Running Conditions Reduced Denaturing (4-20%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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Submitted Jun 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"