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Western blot
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Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)
![Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)](/ps/datasheet/images/ab8978_11.jpg)
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Immunofluorescence - Vimentin antibody [RV202] (ab8978)
![Immunofluorescence - Vimentin antibody [RV202] (ab8978)](/ps/datasheet/images/ab8978_2.jpg)
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Product Name
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Vimentin antibody [RV202] - Neural Stem Cell Marker
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See all Vimentin antibodies (35)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [RV202] to Vimentin - Neural Stem Cell Marker
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Immunogen
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Cytoskeletal vimentin extract of calf lens.
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Reacts with
(species key)
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Hu, Ms, Rat, Chk, Cow, Dog, Goat, Hm, Mk, Zfsh
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Specificity
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This antibody reacts exclusively with vimentin
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Tested applications
(see key)
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Flow Cyt, ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/100 - 1/200.
ICC: 1/20.
ICC/IF: Use at an assay dependent dilution (PMID 20097175).
IHC-Fr: Recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Immunohistochemistry (PFA fixed): 1/1000.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/1000. Detects a band of approximately 57 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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tonsilar lymphoma, cultured bovine lens epithelial cells
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Cellular localization
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Cytoplasmic
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Research areas
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Developmental Biology >> Lineage specification >> Ectoderm
Neuroscience >> Neurology process >> Neuroregeneration >> Neuroregeneration
Cancer >> Tumor biomarkers >> Other
Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Lineage Markers >> Endoderm
Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Intermediate Filaments >> Class III >> Vimentin
Neuroscience >> Cell Type Marker >> Neural Stem Cell marker
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Relevance
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Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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RV202
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Myeloma
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Sp2/0-Ag14
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Vimentin antibody [RV202] - Neural Stem Cell Marker is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Vimentin antibody [RV202] - Neural Stem Cell Marker images, references, products related to ab8978 and other tools.
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Vimentin antibody [RV202] - Neural Stem Cell Marker images:
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 Western blot
One-dimensional immunoblot of BHK-cells incubated with (a) monoclonal desmin antibody, ab8976 and (b) re-incubated with the monoclonal vimentin antibody, ab8978.
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Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)
Ab8978 vimentin staining of a tonsilar lymphoma. Note that the epithelium (at the left) is negative.
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Immunofluorescence - Vimentin antibody [RV202] (ab8978)
Ab8978 vimentin immunofluorescent staining of cultured bovine lens epithelial cells
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Immunocytochemistry/ Immunofluorescence - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)
![Immunocytochemistry/ Immunofluorescence - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)](/ps/datasheet/images/8/ab8978/Vimentin-Primary-antibodies-ab8978-8.jpg)
ab8978 staining Vimentin in bovine chromospheres by ICC/IF (immunocytochemistry/immunofluorescence). Cells were PFA fixed and permeabilized in 0.3% Triton X-100. The primary antibody (1/500) was incubated with the sample for 16 hours at 4°C. An Alexa Fluor® 568-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)
![Immunocytochemistry/ Immunofluorescence - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)](/ps/datasheet/images/8/ab8978/Vimentin-Primary-antibodies-ab8978-10.jpg)
ab8978 staining Vimentin in human immortalized primary epithelial primary cells by ICC/IF (Immunocytochemistry/ Immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with PBS and 0.5% Triton ×100 and blocking with 0.1% BSA + 10% Goat Serum at 250C for 30 minutes was performed. Samples were incubated with primary antibody (1/250: in PBS, 0.1% BSA and 10% Goat Serum) for 12 hours at 4°C. An Alexa Fluor®594-conjugated goat polyclonal to mouse IgG was used undiluted as secondary antibody. This image is a courtesy of Anonymous Abreview
See Abreview
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Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)
![Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)](/ps/datasheet/images/8/ab8978/Vimentin-Primary-antibodies-ab8978-11.jpg)
ab8978 staining Vimentin in human lung tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 5% commercially available blocking agent was performed at 370C for 15 minutes. The sample was incubated with primary antibody (1/250) at 370C for 1 hour. A HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody at 1/1000 dilution. This image is a courtesy of Anonymous Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)
![Immunocytochemistry/ Immunofluorescence - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)](/ps/datasheet/images/8/ab8978/Vimentin-Primary-antibodies-ab8978-12.jpg)
ab8978 staining vimentin in human pancreatic adenocarcinoma cells by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.2% Triton X prior to blocking in 3% BSA for 30 minutes at 24°C. The primary antibody was diluted 1/200 and incubated with the sample for 16 hours at 21°C. Alexa fluor® 488 mouse polyclonal to mouse Ig, diluted 1/300 was used as the secondary antibody This image is courtesy of an Abreview submitted by Jennifer Dembinski
See Abreview
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References for Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)
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This product has been used in: (two most recent references)
Modak C et al. CCN1 is critical for acid-induced esophageal epithelial cell transformation. Biochem Biophys Res Commun 392:533-7 (2010). ICC/IF; Human. PubMed: 20097175
Chai J et al. CCN1 induces a reversible epithelial-mesenchymal transition in gastric epithelial cells. Lab Invest 90:1140-51 (2010). ICC/IF, IHC-P; Rat. PubMed: 20458273
See all 6 publication references for this product.
If you publish research using ab8978 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Vimentin
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Frozen sections) - Vimentin antibody [RV202] - Neural Stem Cell Marker (ab8978)](#ab8978_11.jpg)
![Immunofluorescence - Vimentin antibody [RV202] (ab8978)](#ab8978_2.jpg)
