Anti-Vimentin antibody [SP20], prediluted (ab27608)

Overview

  • Product nameAnti-Vimentin antibody [SP20], prediluted
    See all Vimentin primary antibodies
  • Description
    Rabbit monoclonal [SP20] to Vimentin, prediluted
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human)

  • Positive control
    • Sarcomas.

Properties

Applications

Our Abpromise guarantee covers the use of ab27608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 18703039Incubate for 10 minutes at room temperature.
Flow Cyt Use at an assay dependent concentration. Use Neat



ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration. Predicted molecular weight: 53 kDa.
IHC-P Use at an assay dependent concentration. Do not perform antigen retrieval.

Target

  • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in diseaseCataract 30
  • Sequence similaritiesBelongs to the intermediate filament family.
  • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • FormVimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody [SP20], prediluted images

  • Human melanoma stained with prediluted ab27608.
  • Anti-MDM2 antibody [4B11] (ab16900) at 1/100 dilution + HeLa cell lysate

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Overlay histogram showing HeLa cells stained with ab27608 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab27608, neat) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Vimentin antibody [SP20], prediluted (ab27608)

This product has been referenced in:
  • Yang C  et al. FOXM1 promotes the epithelial to mesenchymal transition by stimulating the transcription of Slug in human breast cancer. Cancer Lett 340:104-12 (2013). WB ; Human . Read more (PubMed: 23856032) »
  • Ahmad A  et al. Phosphoglucose isomerase/autocrine motility factor mediates epithelial-mesenchymal transition regulated by miR-200 in breast cancer cells. Cancer Res 71:3400-9 (2011). WB ; Human . Read more (PubMed: 21389093) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us. The antibody is ready to use and should not be further diluted.Because the antibody is from tissue culture supernatant and we do not routinely determine the concentration. Please do not hesitate to contact me with addition...

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