Anti-Vimentin antibody [V9] (Biotin) (ab79032)

Overview

  • Product name
    Anti-Vimentin antibody [V9] (Biotin)
    See all Vimentin primary antibodies
  • Description
    Mouse monoclonal [V9] to Vimentin (Biotin)
  • Conjugation
    Biotin
  • Specificity
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Horse, Chicken, Hamster, Cow, Cat, Dog, Human, Pig, Monkey, Gerbil
  • Immunogen

    Purified Vimentin from pig eye lens

  • Positive control
    • Human osteosarcoma and other Sarcoma tissues.
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab79032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 for 20 min at RT.
    Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
    • Tissue specificity
      Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
    • Involvement in disease
      Cataract 30
    • Sequence similarities
      Belongs to the intermediate filament family.
    • Domain
      The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    • Post-translational
      modifications
      Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Form
      Vimentin is found in connective tissue and in the cytoskeleton.
    • Alternative names
      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Anti-Vimentin antibody [V9] (Biotin) images

    • ab79032 at 1/100 dilution staining Vimentin in human osteosarcoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of tumor cells. The purified form of this antibody was used for staining.

    References for Anti-Vimentin antibody [V9] (Biotin) (ab79032)

    ab79032 has not yet been referenced specifically in any publications.

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    Suite a notre conversation téléphonique je me suis renseignée a propos du code et je suis prête à faire une exception cette fois-ci et vous le donner.

    Code de remise : xxxxxxxx
    Date d’expiration : ...

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    Merci pour toutes ces informations. Votre protocole me semble sans faute et correspond à la manière dont cet anticorps a été caractérisé. Je pense que vous avez malheureusement reçu un tube ou un lot défectueux. Nos produits sont garantis...

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    Je suis désolé d'apprendre que ab79032 ne vous donne pas de bons résultats en IHC. Nous n'avons pas de date de péremption pour nos produits. Les produits qui doivent être stockés à 4°C sont normalement stables pendant une durée minimale d'un an...

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