Anti-Vimentin antibody [VI-01] (Dyomics® 547) (ab38873)


  • Product nameAnti-Vimentin antibody [VI-01] (Dyomics® 547)
    See all Vimentin primary antibodies
  • Description
    Mouse monoclonal [VI-01] to Vimentin (Dyomics® 547)
  • ConjugationDyomics® 547
  • SpecificityThis antibody is specific for Vimentin. Cross-reactivity was found with smooth muscle desmin.
  • Tested applicationsSuitable for: ICCmore details
  • Species reactivity
    Reacts with all species.
  • Immunogen

    Pellet of pig brain cold stable proteins after depolymerization of microtubules.

  • General notes

    The conjugate is purified by size chromatography and adjusted for direct use.



Our Abpromise guarantee covers the use of ab38873 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC on fixed and permeabilized cells: 1/50.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
    • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
    • Involvement in diseaseCataract 30
    • Sequence similaritiesBelongs to the intermediate filament family.
    • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    • Post-translational
      Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • FormVimentin is found in connective tissue and in the cytoskeleton.
    • Alternative names
      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Anti-Vimentin antibody [VI-01] (Dyomics® 547) images

    • Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with ab38873 anti-Vimentin (VI-01) Dyomics® 547. Nuclei are stained with DAPI (blue).

      Cells were fixed with methanol for 10 minutes at -20°C.

    References for Anti-Vimentin antibody [VI-01] (Dyomics® 547) (ab38873)

    ab38873 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab38873.
    Please use the links above to contact us or submit feedback about this product.