For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Full length native protein (purified) corresponding to Vimentin.
Our Abpromise guarantee covers the use of ab20346 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. Used at a dilution of 1/1000, 1 hr incubation on rat astrocytes (see Abreview).|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.|
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells.
ab91545 - Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Immunofluorescence staining of RBL rat basophiilioc cell line with anti-Vimentin (VI--10). Nuclei are stained with DAPI (blue).
ab20346 at a 1/1000 dilution staining rat astrocyte cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a goat anti-mouse Alexa-Fluor ® 568 (ab175473).
This image is courtesy of an Abreview submitted by Randal Moldrich on 8 February 2006
ab20346 at a 1/500 dilution staining mouse dissociated neural precursor cells by immunocytochemistry. The antibody was incubated with the cells for 1 hour and then detected using a goat anti-mouse Alexa-Fluor 568 (red stain) ab175473>ab175473). The image also shows a blue nuclear counterstain. Confocal image is shown with 2x zoom detail in top right corner.
This image is courtesy of an Abreview submitted by Randal Moldich on 22 February 2006.
Image from Gao MQ et al, J Cell Sci. 2010 Oct 15;123(Pt 20):3507-14. Epub 2010 Sep 14, Fig 3. DOI 10.1242/?jcs.072900
ab20346 used at a 1/1000 dilution in Western Blot.5-20µg of total protein from each sample was loaded.
Overlay histogram showing NIH3T3 cells stained with ab20346 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab20346, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in NIH3T3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.