Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)

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Overview

  • Product name
    Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin)
    See all Vimentin primary antibodies
  • Description
    Mouse monoclonal [VI-RE/1] to Vimentin (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    The antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Pig
  • Immunogen

    Recombinant human vimentin.

  • Positive control
    • ICC/IF: HeLa cells
  • General notes

    Conjugated with R-Phycoerythrin (PE) under optimum conditions.

Properties

Applications

Our Abpromise guarantee covers the use of ab49918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use a concentration of 10 µg/ml.

Working concentrations should be determined by the investigator.





ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificity
    Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in disease
    Cataract 30
  • Sequence similarities
    Belongs to the intermediate filament family.
  • Domain
    The central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
    • Form
      Vimentin is found in connective tissue and in the cytoskeleton.
    • Alternative names
      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) images

    • Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)
      Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)

      ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    • Flow Cytometry - Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)
      Flow Cytometry - Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)Image from Cervelló I et al., PLoS One. 2011;6(6):e21221. Epub 2011 Jun 21. Fig 5.; doi:10.1371/journal.pone.0021221; June 21, 2011, PLoS ONE 6(6): e21221.

      Flow cytometric analysis of Human stromal endometrial somatic stem cells, staining Vimentin with ab49918.

      Gating Strategy: Live cells

    References for Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)

    This product has been referenced in:
    • Cervelló I  et al. Reconstruction of endometrium from human endometrial side population cell lines. PLoS One 6:e21221 (2011). Flow Cyt ; Human . Read more (PubMed: 21712999) »
    • Basu D  et al. Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity. Oncogene : (2010). Flow Cyt . Read more (PubMed: 20498638) »

    See all 2 Publications for this product

    Publishing research using ab49918? Please let us know so that we can cite the reference in this datasheet.

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    Hello!



    I need C of A’s for the following two products:



    Anti-Vimentin, Catalog # AB49918, lot # **

    Mouse IgG1, cat. # AB81200, lot # **



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