Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)
- Datasheet
- Specific References (2)
- Protocols
Overview
- Product nameAnti-Vimentin antibody [VI-RE/1] (Phycoerythrin)
See all Vimentin primary antibodies - DescriptionMouse monoclonal [VI-RE/1] to Vimentin (Phycoerythrin)
- ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
- SpecificityThe antibody VI-RE/1 reacts with human vimentin, a 57 kDa intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.
- Tested applicationsSuitable for: ICC/IF, Flow Cytmore details
- Species reactivityReacts with: Human
Does not react with: Mouse, Pig - Immunogen
Recombinant human vimentin.
- Positive control
- ICC/IF: HeLa cells
- General notes
Conjugated with R-Phycoerythrin (PE) under optimum conditions.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at +4°C.
- Storage bufferPreservative: 15mM Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PuritySize exclusion
- Purification notesPurified antibodies – protein A (G) affinity chromatography before conjugation. Conjugated antibodies –site exclusion chromatography.
- ClonalityMonoclonal
- Clone numberVI-RE/1
- IsotypeIgG1
- Research areas
Associated products
- Compatible Secondaries
- Related Products
Applications
Our Abpromise guarantee covers the use of ab49918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 10 µg/ml. | |
| Flow Cyt | Use a concentration of 10 µg/ml. Working concentrations should be determined by the investigator. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
- FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. - Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
- Involvement in diseaseCataract 30
- Sequence similaritiesBelongs to the intermediate filament family.
- DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex. - Post-translational
modificationsFilament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. - Cellular localizationCytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 7431 Human
- Omim: 193060 Human
- SwissProt: P08670 Human
- Unigene: 455493 Human
- Unigene: 691131 Human
- FormVimentin is found in connective tissue and in the cytoskeleton.
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Alternative names
- CTRCT30 antibody
- Epididymis luminal protein 113 antibody
- FLJ36605 antibody
see all
Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) images
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![Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)](http://a.abcam.com/ps/products/49/ab49918/Images/ab49918-249563-ICCGR112473301.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)ab49918 staining Vimentinn in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab49918 at a working concentration of 10μg/ml (shown in red) and ab185031, Rabbit monoclonal [EP1332Y] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 1/250 overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). -
![Flow Cytometry - Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)](http://a.abcam.com/ps/datasheet/images/49/ab49918/Vimentin-Primary-antibodies-ab49918-2.jpg)
Flow Cytometry - Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)Image from Cervelló I et al., PLoS One. 2011;6(6):e21221. Epub 2011 Jun 21. Fig 5.; doi:10.1371/journal.pone.0021221; June 21, 2011, PLoS ONE 6(6): e21221.Flow cytometric analysis of Human stromal endometrial somatic stem cells, staining Vimentin with ab49918.
Gating Strategy: Live cells
References for Anti-Vimentin antibody [VI-RE/1] (Phycoerythrin) (ab49918)
This product has been referenced in:
- Cervelló I et al. Reconstruction of endometrium from human endometrial side population cell lines. PLoS One 6:e21221 (2011). Flow Cyt ; Human . Read more (PubMed: 21712999) »
- Basu D et al. Evidence for mesenchymal-like sub-populations within squamous cell carcinomas possessing chemoresistance and phenotypic plasticity. Oncogene : (2010). Flow Cyt . Read more (PubMed: 20498638) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"