• Product nameAnti-Vimentin antibody [Vim 3B4]
    See all Vimentin primary antibodies
  • Description
    Mouse monoclonal [Vim 3B4] to Vimentin
  • SpecificityAb28028 recognises Vimentin
  • Tested applicationsSuitable for: WB, IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Human, Xenopus laevis
  • Immunogen

    Full length native protein (purified): Vimentin isolated from bovine eye lens.

  • EpitopeAb28028 reacts with an epitope located in the coil 2 part of the vimentin rod domain.
  • Positive control



Our Abpromise guarantee covers the use of ab28028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Predicted molecular weight: 54 kDa.
IHC-Fr 1/100 - 1/200. The antibody can be used for labeling acetone fixed, frozen sections.
IHC-P 1/100 - 1/200. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in diseaseCataract 30
  • Sequence similaritiesBelongs to the intermediate filament family.
  • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • FormVimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin antibody [Vim 3B4] images

  • ab28028 staining of tonsil by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  • ab28028 staining of tonsil by Immunohistochemistry(Formalin-fixed paraffin-embedded sections)

  •  ab28028 at 1/200 dilution staining vimentin in human lung cancer cells. The Vimentin staining is shown in red. The cells were counterstained with DAPI. Please see accompanying abreview for additional information.

    See Abreview

  • ab28028 staining Vimentin in human fibrosarcoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 1% Triton x100. Samples were incubated with primary antibody (1/100) for 1 hour at 20°C. An Alexa Fluor®488-conjugated donkey monoclonal to mouse IgG (ab150105) was used at dilution at 1/500 as secondary antibody.

    See Abreview

References for Anti-Vimentin antibody [Vim 3B4] (ab28028)

This product has been referenced in:
  • Cui Y  et al. Asparaginyl endopeptidase promotes the invasion and metastasis of gastric cancer through modulating epithelial-to-mesenchymal transition and analysis of their phosphorylation signaling pathways. Oncotarget 7:34356-70 (2016). Human . Read more (PubMed: 27102302) »
  • Venkatesan N  et al. Distribution of a-Gustducin and Vimentin in premature and mature taste buds in chickens. Biochem Biophys Res Commun 479:305-311 (2016). Read more (PubMed: 27639649) »

See all 13 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibrosarcoma)
Specification fibrosarcoma
Fixative Paraformaldehyde
Permeabilization Yes - 1% TX-100

Abcam user community

Verified customer

Submitted Oct 16 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung cancer)
Specification Lung cancer
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C

Dr. Da Zhu

Verified customer

Submitted Mar 26 2009