A synthetic acetylated peptide corresponding to residues surrounding Ser72 of Vimentin
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
A trial size is available to purchase for this antibody.
Our Abpromise guarantee covers the use of ab52944 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100000 - 1/1e+006. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).|
Primary antibody dilution: 1/1000
Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated
Secondary antibody dilution: 1/2500
Blocking & dilution buffer: 5% NFDM/TBST
Lane 1 sample: Vimentin (pS72) phospho peptide
Lane 2 sample: Vimentin non-phospho peptide
Exposure time: 3 minutes