• Product nameAnti-Vimentin (phospho S82) antibody [EP1071Y]
    See all Vimentin primary antibodies
  • Description
    Rabbit monoclonal [EP1071Y] to Vimentin (phospho S82)
  • Specificityab52943 only detects Vimentin phosphorylated at Serine 82.
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic acetylated peptide corresponding to residues surrounding Ser82 of Vimentin

  • Positive control
    • AP treated and untreated HeLa + Calyculin A cell lysate.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.



Our Abpromise guarantee covers the use of ab52943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesWB: 1/500000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

    Is unsuitable for Flow Cyt, IHC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
    • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
    • Involvement in diseaseCataract 30
    • Sequence similaritiesBelongs to the intermediate filament family.
    • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
      The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
    • Post-translational
      Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • FormVimentin is found in connective tissue and in the cytoskeleton.
    • Alternative names
      • CTRCT30 antibody
      • Epididymis luminal protein 113 antibody
      • FLJ36605 antibody
      • HEL113 antibody
      • VIM antibody
      • VIME_HUMAN antibody
      • Vimentin antibody
      see all

    Anti-Vimentin (phospho S82) antibody [EP1071Y] images

    • Primary antibody dilution: 1/1000

      Secondary antibody: goat anti-rabbit IgG, (H+L), peroxidase conjugated

      Secondary antibody dilution: 1/2500

      Blocking & dilution buffer: 5% NFDM/TBST

      Lane 1 sample: Vimentin (pS82) phospho peptide

      Lane 2 sample: Vimentin non-phospho peptide

      Exposure time: 3 minutes

    • All lanes : Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943) at 1/500000 dilution

      Lane 1 : HeLa cell lysate AP treated
      Lane 2 : HeLa cell lysate (AP untreated) + Calyculin A

      Lysates/proteins at 10 µg per lane.

      goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 54 kDa
      Observed band size : 54 kDa

    References for Anti-Vimentin (phospho S82) antibody [EP1071Y] (ab52943)

    This product has been referenced in:
    • Goto H  et al. Phospho-Specific Antibody Probes of Intermediate Filament Proteins. Methods Enzymol 568:85-111 (2016). Read more (PubMed: 26795468) »
    • Haolong C  et al. Enterovirus 71 VP1 activates calmodulin-dependent protein kinase II and results in the rearrangement of vimentin in human astrocyte cells. PLoS One 8:e73900 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24073199) »

    See all 3 Publications for this product

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