Anti-Vimentin (phospho S83) antibody (ab79189)

Overview

  • Product nameAnti-Vimentin (phospho S83) antibody
    See all Vimentin primary antibodies
  • Description
    Rabbit polyclonal to Vimentin (phospho S83)
  • Specificityab79189 detects endogenous levels of Vimentin only when phosphorylated at serine 83.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human Vimentin around the phosphorylation site of serine 83 (Q-D-SP-V-D).

  • Positive control
    • 293 cytoplasmic lysate treated with paclitaxel (1 µM, 24 hours); human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 54 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

  • FunctionVimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
    Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • Tissue specificityHighly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
  • Involvement in diseaseCataract 30
  • Sequence similaritiesBelongs to the intermediate filament family.
  • DomainThe central alpha-helical coiled-coil rod region mediates elementary homodimerization.
    The [IL]-x-C-x-x-[DE] motif is a proposed target motif for cysteine S-nitrosylation mediated by the iNOS-S100A8/A9 transnitrosylase complex.
  • Post-translational
    modifications
    Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33.
    O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
    S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • FormVimentin is found in connective tissue and in the cytoskeleton.
  • Alternative names
    • CTRCT30 antibody
    • Epididymis luminal protein 113 antibody
    • FLJ36605 antibody
    • HEL113 antibody
    • VIM antibody
    • VIME_HUMAN antibody
    • Vimentin antibody
    see all

Anti-Vimentin (phospho S83) antibody images

  • All lanes : Anti-Vimentin (phospho S83) antibody (ab79189) at 1/500 dilution

    Lane 1 : 293 cells treated with paclitaxel (1 µM, 24 hours)
    Lane 2 : 293 cells treated with paclitaxel (1 µM, 24 hours) with immunizing peptide at 10 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 54 kDa
    Observed band size : 54 kDa
  • ab79189, at a 1/50 dilution, staining Vimentin in formalin fixed, paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
    Left image: no immunizing peptide.
    Right image: with immunizing phospho peptide.

References for Anti-Vimentin (phospho S83) antibody (ab79189)

ab79189 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab79189.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"