Overview

  • Product name
  • Description
    Rabbit polyclonal to Vinexin
  • Tested applications
    Suitable for: WB, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide derived from an internal domain of mouse Vinexin.

Properties

Applications

Our Abpromise guarantee covers the use of ab89654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/2000. Predicted molecular weight: 75 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    Vinexin is a focal adhesion protein with a sorbin homology (SoHo) domain as well as 3 SH3 domains. It exists as at least three splice variants - alpha, beta and gamma. It is thought to play a key role in cell adhesion, cell spreading and migration and cytoskeletal organisation. It has also been implicated signaling due to its interaction with the extracellular signal-regulated kinase ERK. Binds to vinculin at sites of cell-cell contact.
  • Cellular localization
    Localized at cell-extracellular matrix junctions. Both isoforms were localized at focal adhesion and cell-cell adhesion sites, vinexin beta was also found in the nucleus.
  • Database links
  • Alternative names
    • SCAM 1 antibody
    • SCAM1 antibody
    • SH3 containing adapter molecule 1 antibody
    • SH3 domain containing adapter molecule 1 antibody
    • SH3D4 antibody
    • Sorbin and SH3 domain containing 3 antibody
    • Sorbin and SH3 domain containing protein 3 antibody
    • Sorbs3 antibody
    • Vinexin alpha antibody
    • Vinexin antibody
    • Vinexin beta (SH3 containing adaptor molecule 1) antibody
    see all

References

ab89654 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification
Mouse Embryonic Fibroblast (MEF)
Fixative
Formaldehyde
Permeabilization
Yes - triton
Username

Abcam user community

Verified customer

Submitted Aug 16 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa cell line ( cervical cancer))
Specification
HeLa cell line ( cervical cancer)
Fixative
Formaldehyde
Permeabilization
Yes - triton
Username

Abcam user community

Verified customer

Submitted Aug 16 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up