Overview

  • Product nameAnti-VIP antibody
    See all VIP primary antibodies
  • Description
    Rabbit polyclonal to VIP
  • Specificityab22736 reacts with Vasoactive Intestinal (poly)Peptide (VIP).
  • Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Dog, Human, Pig
  • Immunogen

    Full length native protein (purified) (Pig) conjugated to KLH by a Glutaraldehyde linker.

  • Positive control
    • Human, rat and porcine colon and spinal cord.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
  • Application notesIHC-Fr: 1/400 - 1/1600.
    IHC-P: 1/400 - 1/1600.
    Notes: Dilute to working strength with PBS containing 0.1% w/v sodium azide.
    Store diluted antibodies at 2-4°C(do not freeze) and use within 1 month.
    using overnight incubation and indirect immunofluorescence procedure.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-VIP antibody images

    • ab22736 staining VIP in jejunum tissue section of pig by Immunohistochemistry (frozen sections). Tissue underwent fixation in paraformaldehyde and incubated with primary antibody, diluted 1/200 for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit, dilution 1/400 was used as the secondary.

      See Abreview

    • ab22736 staining VIP in sheep abomasum tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody was diluted 1/1200 and incubated with sample for 24 hours. A FITC-conjugated goat polyclonal (IgG whole molecule) to rabbit IgG was diluted 1/400 and used as secondary. 

    References for Anti-VIP antibody (ab22736)

    ab22736 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Pig Tissue sections (jejunum)
    Specification jejunum
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    Submitted Nov 06 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Sheep Tissue sections (abomasum)
    Specification abomasum
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    Submitted Oct 22 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Dog Tissue sections (duodenum)
    Specification duodenum
    Fixative Paraformaldehyde
    Permeabilization No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    Submitted Jun 22 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"