Overview

  • Product name
  • Description
    Rabbit polyclonal to VIP
  • Specificity
    ab22736 reacts with Vasoactive Intestinal (poly)Peptide (VIP).
  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Sheep, Dog, Human, Pig
  • Immunogen

    Full length native protein (purified) (Pig) conjugated to KLH by a Glutaraldehyde linker.

  • Positive control
    • Human, rat and porcine colon and spinal cord.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • Purity
    Whole antiserum
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22736 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
IHC-P
  • Application notes
    IHC-Fr: 1/400 - 1/1600.
    IHC-P: 1/400 - 1/1600.
    Notes: Dilute to working strength with PBS containing 0.1% w/v sodium azide.
    Store diluted antibodies at 2-4°C(do not freeze) and use within 1 month.
    using overnight incubation and indirect immunofluorescence procedure.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-VIP antibody images

    • ab22736 staining VIP in jejunum tissue section of pig by Immunohistochemistry (frozen sections). Tissue underwent fixation in paraformaldehyde and incubated with primary antibody, diluted 1/200 for 24 hours. A Texas Red® conjugated goat polyclonal to rabbit, dilution 1/400 was used as the secondary.

      See Abreview

    • ab22736 staining VIP in sheep abomasum tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in paraformaldehyde without undergoing permeabilization and blocking steps. The primary antibody was diluted 1/1200 and incubated with sample for 24 hours. A FITC-conjugated goat polyclonal (IgG whole molecule) to rabbit IgG was diluted 1/400 and used as secondary. 

    References for Anti-VIP antibody (ab22736)

    This product has been referenced in:
    • Hallett PJ  et al. a-Synuclein overexpressing transgenic mice show internal organ pathology and autonomic deficits. Neurobiol Dis 47:258-67 (2012). Read more (PubMed: 22549133) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (jejunum)
    Specification
    jejunum
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    Submitted Nov 06 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Sheep Tissue sections (abomasum)
    Specification
    abomasum
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    Submitted Oct 22 2008

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Dog Tissue sections (duodenum)
    Specification
    duodenum
    Fixative
    Paraformaldehyde
    Permeabilization
    No
    Username

    Prof. Marcin Arciszewski

    Verified customer

    Submitted Jun 22 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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