Overview

  • Product nameAnti-VIP antibody
    See all VIP primary antibodies
  • Description
    Rabbit polyclonal to VIP
  • SpecificityThe antiserum supplied has been evaluated for its ability to stain ganglion cells in both the superficial and deep plexus of the wall of the human small bowel.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Cat, Dog, Human
  • Immunogen

    Synthetic porcine vasoactive intestinal peptide,

    SAKKYLESLIX

    (amino acids 18-28), bound to KLH with carbodiimide.

  • Positive control
    • Small bowel tissue with plexus attached. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HCT116.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • PurityWhole antiserum
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab78536 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.
IHC-Fr 1/500 - 1/1000.
ICC/IF 1/200.

Target

Anti-VIP antibody images

  • IHC image of ab78536 staining in mouse intestine formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78536, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab78536 stained HCT116 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab78536 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-VIP antibody (ab78536)

This product has been referenced in:
  • De Vadder F  et al. Vasoactive intestinal peptide is a local mediator in a gut-brain neural axis activating intestinal gluconeogenesis. Neurogastroenterol Motil 27:443-8 (2015). IHC-P ; Rat . Read more (PubMed: 25586379) »
  • Raghavan S  et al. Successful implantation of bioengineered, intrinsically innervated, human internal anal sphincter. Gastroenterology 141:310-9 (2011). IHC-P ; Human . Read more (PubMed: 21463628) »

See all 2 Publications for this product

Product Wall

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I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by pass...

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Thank you for contacting Abcam regarding ab78536.

I am sorry that you have been experiencing difficulties with this antibody in your experiments. I have reviewed the protocol information you provided and I agree that this antibody does not a...

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Vielen Dank für Ihre Anfrage. Leider wurde die genaue Konzentration dieses Antikörpers nicht gemessen. Es handelt sich hierbei um ein un-aufgereinigtes Antiserum und es kann höchstens die Gesamtproteinkonzentration gemessen werden. Als genereller Anhal...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"