Overview

  • Product nameAnti-Visfatin antibody [4D5]
    See all Visfatin primary antibodies
  • Description
    Mouse monoclonal [4D5] to Visfatin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) purified from E. coli

  • Positive control
    • 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab71505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionCatalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • Tissue specificityExpressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • PathwayCofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • Sequence similaritiesBelongs to the NAPRTase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110035O14Rik antibody
    • AI314458 antibody
    • AI480535 antibody
    • DKFZP666B131 antibody
    • EC 2.4.2.12 antibody
    • MGC117256 antibody
    • NAmPRTase antibody
    • Nampt antibody
    • NAMPT_HUMAN antibody
    • Nicotinamide phosphoribosyltransferase antibody
    • PBEF 1 antibody
    • PBEF antibody
    • PBEF1 antibody
    • Pre B cell colony enhancing factor 1 antibody
    • Pre B cell colony enhancing factor antibody
    • Pre B cell enhancing factor antibody
    • Pre-B cell-enhancing factor antibody
    • Pre-B-cell colony-enhancing factor 1 antibody
    • VF antibody
    • Visfatin antibody
    see all

Anti-Visfatin antibody [4D5] images



  • Predicted band size : 56 kDa

    (Top) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : 293T cell lysate (30ug)

    (Bottom) The cell lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Visfatin (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

    Lane 1 : SW480 cell lysate (40ug)

  • ICC/IF analysis of Visfatin in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Visfatin antibody (1:100) with goat anti-mouse IgG-Alexa fluor® 488 conjugate (Green).

References for Anti-Visfatin antibody [4D5] (ab71505)

ab71505 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab71505.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"