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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PBEF.
(Peptide available as ab45889.)
Our Abpromise guarantee covers the use of ab45890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.
Use a concentration of 10 µg/ml.
|WB||1/250. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).|
ICC/IF image of ab45890 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab45890 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab45890 staining PBEF in rat brain tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/1000 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgG was used as secondary, at 1/1000 dilution.
This antibody produces a nice staining in rat cortical neurons. Some tracts of fibers were stained as well. The picture shows the staining obtained in cortical neurons.
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