Overview

  • Product nameAnti-Visfatin antibody
    See all Visfatin primary antibodies
  • Description
    Rabbit polyclonal to Visfatin
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Chicken, Human, Chimpanzee, Monkey
    Predicted to work with: Mouse, Rat, Horse, Cow
  • Immunogen

    Synthetic peptide (Human) corresponding to a region between residues 30-80 of PBEF (NP_005737.1).

  • Positive control
    • Human, Mouse or Rat Skeletal muscle lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72128 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • FunctionCatalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway.
  • Tissue specificityExpressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues.
  • PathwayCofactor biosynthesis; NAD(+) biosynthesis; nicotinamide D-ribonucleotide from 5-phospho-alpha-D-ribose 1-diphosphate and nicotinamide: step 1/1.
  • Sequence similaritiesBelongs to the NAPRTase family.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1110035O14Rik antibody
    • AI314458 antibody
    • AI480535 antibody
    • DKFZP666B131 antibody
    • EC 2.4.2.12 antibody
    • MGC117256 antibody
    • NAmPRTase antibody
    • Nampt antibody
    • NAMPT_HUMAN antibody
    • Nicotinamide phosphoribosyltransferase antibody
    • PBEF 1 antibody
    • PBEF antibody
    • PBEF1 antibody
    • Pre B cell colony enhancing factor 1 antibody
    • Pre B cell colony enhancing factor antibody
    • Pre B cell enhancing factor antibody
    • Pre-B cell-enhancing factor antibody
    • Pre-B-cell colony-enhancing factor 1 antibody
    • VF antibody
    • Visfatin antibody
    see all

Anti-Visfatin antibody images

  • All lanes : Anti-Visfatin antibody (ab72128) at 1/5000 dilution

    Lane 1 : Pre immune sera
    Lane 2 : Human skeletal muscle lysate
    Lane 3 : Mouse skeletal muscle lysate
    Lane 4 : Rat skeletal muscle lysate


    Predicted band size : 56 kDa
    Observed band size : 56 kDa

References for Anti-Visfatin antibody (ab72128)

ab72128 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (U87 human glioblastoma cells)
Loading amount 8000 cells
Specification U87 human glioblastoma cells
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Desire Ngoga

Verified customer

Submitted Jun 14 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"