Anti-Vitamin D Binding protein antibody [19E8] (ab23485)

Overview

  • Product nameAnti-Vitamin D Binding protein antibody [19E8]
    See all Vitamin D Binding protein primary antibodies
  • Description
    Mouse monoclonal [19E8] to Vitamin D Binding protein
  • Tested applicationsSuitable for: ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Vitamin D Binding Protein isolated from human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab23485 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionMultifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBP antibody
    • DBP/GC antibody
    • GC antibody
    • Gc globulin antibody
    • Gc-globulin antibody
    • GRD3 antibody
    • Group specific component antibody
    • Group specific component vitamin D binding protein antibody
    • Group-specific component antibody
    • hDBP antibody
    • VDB antibody
    • VDBG antibody
    • VDBP antibody
    • Vitamin D binding alpha globulin antibody
    • Vitamin D-binding protein antibody
    • VTDB_HUMAN antibody
    see all

Anti-Vitamin D Binding protein antibody [19E8] images

  • IHC image of ab23485 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23485, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Vitamin D Binding protein antibody [19E8] (ab23485)

ab23485 has not yet been referenced specifically in any publications.

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