• Product nameAnti-Vitamin D Binding protein antibody
    See all Vitamin D Binding protein primary antibodies
  • Description
    Rabbit polyclonal to Vitamin D Binding protein
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Vitamin D Binding Protein.

    (Peptide available as ab71146.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: human liver and human ovary IHC-P: Human kidney FFPE tissue sections.


Associated products


Our Abpromise guarantee covers the use of ab65636 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).


  • FunctionMultifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.
  • Sequence similaritiesBelongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • DBP antibody
    • DBP/GC antibody
    • GC antibody
    • Gc globulin antibody
    • Gc-globulin antibody
    • GRD3 antibody
    • Group specific component antibody
    • Group specific component vitamin D binding protein antibody
    • Group-specific component antibody
    • hDBP antibody
    • VDB antibody
    • VDBG antibody
    • VDBP antibody
    • Vitamin D binding alpha globulin antibody
    • Vitamin D-binding protein antibody
    • VTDB_HUMAN antibody
    see all

Anti-Vitamin D Binding protein antibody images

  • IHC image of Vitamin D Binding Protein staining in human kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab65636, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

  • All lanes : Anti-Vitamin D Binding protein antibody (ab65636) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Human ovary tissue lysate - total protein (ab30222)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 53 kDa
    Observed band size : 53 kDa

    Exposure time : 1 minute

  • developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 53 kDa

    Exposure time : 1 minute
  • ICC/IF image of ab65636 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65636, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Vitamin D Binding protein antibody (ab65636)

This product has been referenced in:
  • Ackermann AM  et al. Integration of ATAC-seq and RNA-seq identifies human alpha cell and beta cell signature genes. Mol Metab 5:233-44 (2016). Human . Read more (PubMed: 26977395) »
  • Tao W  et al. Hormonal induction and roles of Disabled-2 in lactation and involution. PLoS One 9:e110737 (2014). WB ; Mouse . Read more (PubMed: 25360623) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Pig Tissue lysate - whole (endometrial tissue lysate)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 25 µg
Specification endometrial tissue lysate
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C

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Submitted Jul 28 2015