Anti-Vitamin D Receptor antibody (ab82566)

Overview

  • Product nameAnti-Vitamin D Receptor antibody
    See all Vitamin D Receptor primary antibodies
  • Description
    Rabbit polyclonal to Vitamin D Receptor
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Chicken, Cow, Pig, Zebrafish
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Vitamin D Receptor .

    (Peptide available as ab90849.)

  • Positive control
    • This antibody gave a positive signal in the following human tissue lysates: Skin; Thyroid; Small intestine; Bone marrow; Kidney.

Properties

Applications

Our Abpromise guarantee covers the use of ab82566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 48 kDa).

Target

  • FunctionNuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
  • Involvement in diseaseDefects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 25 dihydroxyvitamin D3 receptor antibody
    • 1 antibody
    • 1,25 dihydroxyvitamin D3 receptor antibody
    • 1,25-@dihydroxyvitamin D3 receptor antibody
    • 25-dihydroxyvitamin D3 receptor antibody
    • Member 1 antibody
    • NR1I1 antibody
    • Nuclear receptor subfamily 1 group I member 1 antibody
    • PPP1R163 antibody
    • Protein phosphatase 1, regulatory subunit 163 antibody
    • VDR antibody
    • VDR_HUMAN antibody
    • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
    • Vitamin D hormone receptor antibody
    • Vitamin D nuclear receptor variant 1 antibody
    • Vitamin D receptor antibody
    • Vitamin D3 receptor antibody
    see all

Anti-Vitamin D Receptor antibody images

  • All lanes : Anti-Vitamin D Receptor antibody (ab82566) at 1 µg/ml

    Lane 1 : Human skin tissue lysate - total protein (ab30166)
    Lane 2 : Human thyroid normal tissue lysate - total protein (ab29636)
    Lane 3 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 4 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
    Lane 5 : Human kidney tissue lysate - total protein (ab30203)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 54 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 94 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-Vitamin D Receptor antibody (ab82566) at 1 µg/ml + Recombinant Human Vitamin D Receptor protein (ab82068) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 seconds

References for Anti-Vitamin D Receptor antibody (ab82566)

ab82566 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. This antibody can be used with serum samples as long as your serum samples contain vitamin D at detectable level. You would need to optimise the protocol to obtain a lysate suitable for loading in the WB gel. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"