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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
Overview
Product name
Anti-Vitamin D Receptor antibody - ChIP Grade
See all Vitamin D Receptor products (15) ...
Description
Rabbit polyclonal to Vitamin D Receptor - ChIP Grade
Tested applications
ICC/IF, IHC-Fr, Gel supershift assays, IP, WB, IHC-P, ChIPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: EEHSKQYRCLSFQPECSMK, corresponding to amino acids 395-413 of Human Vitamin D Receptor .
EEHSKQYRCL SFQPECSMK
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.05% sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cancer >> Signal transduction >> Nuclear signaling >> Nuclear hormone receptors >> Other
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Vitamin D Receptor
Signal Transduction >> Signaling Pathway >> Nuclear Signaling >> Nuclear Hormone Receptors >> Vitamin D Receptor
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Zinc Finger
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vitamin D Receptor antibody - ChIP Grade (ab3508)
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Immunocytochemistry/ Immunofluorescence - Vitamin D Receptor antibody - ChIP Grade (ab3508)
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Immunohistochemistry (Frozen sections) - Vitamin D Receptor antibody - ChIP Grade (ab3508)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration.
IHC-Fr: Use at an assay dependent concentration.
GSA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100Detects a band of approximately 53 kDa (predicted molecular weight: 48 kDa).(1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).)
IHC-P: 1/2000 - 1/4000.
ChIP: Use at an assay dependent dilution. (PubMed: 17244627Use at an assay dependent dilution.)
Target
Function
Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
Involvement in disease
Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
Sequence similarities
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP11473
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Vitamin D3 receptor antibody
- 1 25 dihydroxyvitamin D3 receptor antibody
- 1 antibody
see all
Database links
- Entrez Gene: 7421 Human
- Entrez Gene: 22337 Mouse
- Entrez Gene: 24873 Rat
- Omim: 601769 Human
- SwissProt: P11473 Human
- SwissProt: P48281 Mouse
- SwissProt: P13053 Rat
- Unigene: 524368 Human
see all
Anti-Vitamin D Receptor antibody - ChIP Grade images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Vitamin D Receptor antibody - ChIP Grade (ab3508)
Ab3508 staining human normal jejunum. Staining is localized to the nucleus.
Left panel: with primary antibody at 1/2000. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Immunocytochemistry/ Immunofluorescence - Vitamin D Receptor antibody - ChIP Grade (ab3508)
ab3508 staining the Vitamin D Receptor in mVDR-transfected and untransfected mouse NIH/3T3 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with 1% BSA/5%HS for 30 minutes at 20°C. The sample was incubated with the primary antibody (1/50 in 1% BSA/5% HS in 1xPBS) for 1 hour 30 minutes at 20°C. A Cy3®-conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary.
This image is courtesy of an Abreview submitted by Dr A Uhmann
Immunohistochemistry (Frozen sections) - Vitamin D Receptor antibody - ChIP Grade (ab3508)
ab3508 at a 1/2000 dilution staining Vitamin D Receptor in whole rat embryo tissue sections by Immunohistochemistry (frozen sections) incubated for 16 hours at 25°C. Samples fixed in 4% PFA prior to cutting at 30µm thickness. Blocked with 5% serum for 1 hour at 25°C. Secondary used at 1/200 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.
This image is courtesy of an anonymous abreview.
References for Anti-Vitamin D Receptor antibody - ChIP Grade (ab3508)
This product has been referenced in:
- Thorne JLet al. Epigenetic control of a VDR-governed feed-forward loop that regulates p21(waf1/cip1) expression and function in non-malignant prostate cells. Nucleic Acids Res 39:2045-56 (2011). ChIP; Human.Read more (PubMed: 21088000) »
- Heikkinen Set al. Nuclear hormone 1{alpha},25-dihydroxyvitamin D3 elicits a genome-wide shift in the locations of VDR chromatin occupancy. Nucleic Acids Res : (2011). CHIPseq.Read more (PubMed: 21846776) »
See all 6 publications for this product
Publishing research using ab3508? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"