Anti-Vitamin D Receptor antibody - ChIP Grade (ab3508)

Overview

  • Product nameAnti-Vitamin D Receptor antibody - ChIP Grade
    See all Vitamin D Receptor primary antibodies
  • Description
    Rabbit polyclonal to Vitamin D Receptor - ChIP Grade
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, Gel supershift assays, IP, WB, CHIPseq, IHC-P, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
    Predicted to work with: Cow, Pig, Zebrafish, Saguinus oedipus
  • Immunogen

    Synthetic peptide corresponding to Human Vitamin D Receptor aa 395-413.
    Sequence:

    EEHSKQYRCLSFQPECSMK

Properties

Applications

Our Abpromise guarantee covers the use of ab3508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Gel supershift assays Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB 1/100. Detects a band of approximately 53 kDa (predicted molecular weight: 48 kDa). 1/100. Detects a band of approximately 53 kDa in COS-7 cells transfected with the human gene (predicted molecular weight: 48 kDa). This antibody supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements).
CHIPseq Use at an assay dependent concentration. PubMed: 21846776
IHC-P 1/2000 - 1/4000.
ChIP Use at an assay dependent concentration. PubMed: 17244627Use at an assay dependent dilution.

Target

  • FunctionNuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
  • Involvement in diseaseDefects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
  • Sequence similaritiesBelongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • DomainComposed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1 25 dihydroxyvitamin D3 receptor antibody
    • 1 antibody
    • 1,25 dihydroxyvitamin D3 receptor antibody
    • 1,25-@dihydroxyvitamin D3 receptor antibody
    • 25-dihydroxyvitamin D3 receptor antibody
    • Member 1 antibody
    • NR1I1 antibody
    • Nuclear receptor subfamily 1 group I member 1 antibody
    • PPP1R163 antibody
    • Protein phosphatase 1, regulatory subunit 163 antibody
    • VDR antibody
    • VDR_HUMAN antibody
    • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
    • Vitamin D hormone receptor antibody
    • Vitamin D nuclear receptor variant 1 antibody
    • Vitamin D receptor antibody
    • Vitamin D3 receptor antibody
    see all

Anti-Vitamin D Receptor antibody - ChIP Grade images

  • Ab3508 staining Human normal jejunum. Staining is localized to the nucleus.
    Left panel: with primary antibody at 1/2000. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer, citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

  • ab3508 staining the Vitamin D Receptor in mVDR-transfected and untransfected Mouse NIH/3T3 cells by ICC/IF (immunocytochemistry/immunofluorescence). Cells were paraformaldehyde fixed, permeabilized with Triton X-100 and blocked with 1% BSA/5%HS for 30 minutes at 20°C. The sample was incubated with the primary antibody (1/50 in 1% BSA/5% HS in 1xPBS) for 1 hour 30 minutes at 20°C. A Cy3®-conjugated goat anti-rabbit polyclonal (1/200) was used as the secondary.

    See Abreview

  • ab3508 at a 1/2000 dilution staining Vitamin D Receptor in whole Rat embryo tissue sections by Immunohistochemistry (frozen sections) incubated for 16 hours at 25°C. Samples fixed in 4% PFA prior to cutting at 30µm thickness. Blocked with 5% serum for 1 hour at 25°C. Secondary used at 1/200 polyclonal Goat anti-rabbit conjugated to Alexa Fluor 488.

    See Abreview

References for Anti-Vitamin D Receptor antibody - ChIP Grade (ab3508)

This product has been referenced in:
  • Zerr P  et al. Vitamin D receptor regulates TGF-ß signalling in systemic sclerosis. Ann Rheum Dis N/A:N/A (2014). Human . Read more (PubMed: 24448349) »
  • Bergadà L  et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest 94:608-22 (2014). Read more (PubMed: 24732451) »

See all 16 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH/3T3, untransfected, mVDR-transfected)
Specification NIH/3T3, untransfected, mVDR-transfected
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100
Blocking step 1% BSA, 5% HS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Miss. A Uhmann

Verified customer

Submitted Dec 03 2009

Thank you for contacting us.

Since this is a whole antiserum antibody, the concentration has not been determined. Typically the total protein concentration for these antibodies can range from 1 to 10 mg/ml (you could split the difference and...

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Thank you very much for contacting us with your enquiry.

Ab3508 is an unpurified whole serum antibody, and as there are other proteins in the serum the concentration of specific IgG can not be measured. This antibody has been used in ChIP in ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Whole embryo)
Specification Whole embryo
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Dec 14 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - nuclear (NIH/3T3, untransfected, VDR-transfected)
Loading amount 10 µg
Specification NIH/3T3, untransfected, VDR-transfected
Gel Running Conditions Reduced Denaturing (10%)
Blocking step I-Block as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Username

Miss. A Uhmann

Verified customer

Submitted Dec 01 2009

Thank you for your enquiry. The resources I have available indicate that 1 ul of ab3508 should be a sufficient amount for immunoprecipitation assays. I would recommend using a minimum of 100 ug of sample. I hope this information helps, please do...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"