Recombinant
RabMAb

Anti-Vitamin D Receptor antibody [EPR4552] (ab109234)

Overview

  • Product name
    Anti-Vitamin D Receptor antibody [EPR4552]
    See all Vitamin D Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR4552] to Vitamin D Receptor
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Vitamin D Receptor .
    (Peptide available as ab198626)

  • Positive control
    • WB: HeLa, T-47D and SKBR-3 cell lysates and rat and mouse kidney tissue lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Vitamin D Receptor peptide (ab198626).

For unpurified use at 1/1000 - 1/10000.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function
      Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
    • Involvement in disease
      Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
    • Sequence similarities
      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain
      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 25 dihydroxyvitamin D3 receptor antibody
      • 1 antibody
      • 1,25 dihydroxyvitamin D3 receptor antibody
      • 1,25-@dihydroxyvitamin D3 receptor antibody
      • 25-dihydroxyvitamin D3 receptor antibody
      • Member 1 antibody
      • NR1I1 antibody
      • Nuclear receptor subfamily 1 group I member 1 antibody
      • PPP1R163 antibody
      • Protein phosphatase 1, regulatory subunit 163 antibody
      • VDR antibody
      • VDR_HUMAN antibody
      • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
      • Vitamin D hormone receptor antibody
      • Vitamin D nuclear receptor variant 1 antibody
      • Vitamin D receptor antibody
      • Vitamin D3 receptor antibody
      see all

    Anti-Vitamin D Receptor antibody [EPR4552] images

    • Anti-Vitamin D Receptor antibody [EPR4552] (ab109234) at 1/5000 dilution (purified) + HeLa cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-Vitamin D Receptor antibody [EPR4552] (ab109234) at 1/5000 dilution (purified) + Mouse kidney tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-Vitamin D Receptor antibody [EPR4552] (ab109234) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Vitamin D Receptor antibody [EPR4552] (ab109234) at 1/1000 dilution (unpurified)

      Lane 1 : T47D cell lysate
      Lane 2 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa

    References for Anti-Vitamin D Receptor antibody [EPR4552] (ab109234)

    This product has been referenced in:
    • Hu H  et al. Effect of 1,25(OH)2D3 on transdifferentiation of rat renal tubular epithelial cells induced by high glucose. Biomed Rep 5:699-704 (2016). WB ; Rat . Read more (PubMed: 28101343) »
    • Lungchukiet P  et al. Suppression of epithelial ovarian cancer invasion into the omentum by 1a,25-dihydroxyvitamin D3 and its receptor. J Steroid Biochem Mol Biol 148:138-47 (2015). Read more (PubMed: 25448740) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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