Recombinant
RabMAb

Anti-Vitamin D Receptor antibody [EPR4552] (ab109234)

Overview

  • Product name
    Anti-Vitamin D Receptor antibody [EPR4552]
    See all Vitamin D Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR4552] to Vitamin D Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ChIPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Vitamin D Receptor. The exact sequence is proprietary.
    (Peptide available as ab198626)

  • Positive control
    • WB: HeLa, T-47D and SKBR-3 cell lysates and rat and mouse kidney tissue lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).Can be blocked with Vitamin D Receptor peptide (ab198626).

For unpurified use at 1/1000 - 1/10000.

IP 1/30.
ChIP Use at an assay dependent concentration.

Use at 5μg

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis.
    • Involvement in disease
      Defects in VDR are the cause of rickets vitamin D-dependent type 2A (VDDR2A) [MIM:277440]. A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets.
    • Sequence similarities
      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • Domain
      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1 25 dihydroxyvitamin D3 receptor antibody
      • 1 antibody
      • 1,25 dihydroxyvitamin D3 receptor antibody
      • 1,25-@dihydroxyvitamin D3 receptor antibody
      • 25-dihydroxyvitamin D3 receptor antibody
      • Member 1 antibody
      • NR1I1 antibody
      • Nuclear receptor subfamily 1 group I member 1 antibody
      • PPP1R163 antibody
      • Protein phosphatase 1, regulatory subunit 163 antibody
      • VDR antibody
      • VDR_HUMAN antibody
      • Vitamin D (1,25- dihydroxyvitamin D3) receptor antibody
      • Vitamin D hormone receptor antibody
      • Vitamin D nuclear receptor variant 1 antibody
      • Vitamin D receptor antibody
      • Vitamin D3 receptor antibody
      see all

    Images

    • Chromatin was prepared from T-47D cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25 µg of chromatin, 2 µg of ab223850 (blue), and 20 µL of protein A/G sepharose beads slurry (10 µL of sepharose A beads + 10 µL of sepharose G beads). 5 μg of rabbit normal IgG was added to the beads as a control sample (yellow). The immunoprecipitated DNA was quantified by real time PCR (SYBR Green chemistry) with primers to c-Fos.

    • Lane 1 (input): T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate, 10 μg
      Lane 2(+): T-47D whole cell lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109234 in T-47D whole cell lysate

      Ab109234 immunoprecipitating Vitamin D receptor in T-47D whole cell lysates. Capture antibody was used at a 1/30 dilution (2 μg in 0.35 mg lysates). For western blotting, primary antibody was used as ab109234 at 1/1000 dilution (0.62 μg/mL). Ab131366 VeriBlot for IP (HRP) was used as the secondary antibody at 1/5000 dilution.

    • Anti-Vitamin D Receptor antibody [EPR4552] (ab109234) at 1/5000 dilution (purified) + HeLa cell lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-Vitamin D Receptor antibody [EPR4552] (ab109234) at 1/5000 dilution (purified) + Mouse kidney tissue lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Anti-Vitamin D Receptor antibody [EPR4552] (ab109234) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • All lanes : Anti-Vitamin D Receptor antibody [EPR4552] (ab109234) at 1/1000 dilution (unpurified)

      Lane 1 : T47D cell lysate
      Lane 2 : SKBR-3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 48 kDa
      Observed band size: 48 kDa

    References

    This product has been referenced in:
    • Loughran G  et al. Stop codon readthrough generates a C-terminally extended variant of the human vitamin D receptor with reduced calcitriol response. J Biol Chem 293:4434-4444 (2018). Read more (PubMed: 29386352) »
    • Yang L  et al. 1,25(OH)2D3 treatment attenuates high glucose-induced peritoneal epithelial to mesenchymal transition in mice. Mol Med Rep 16:3817-3824 (2017). Read more (PubMed: 28765896) »

    See all 6 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109234.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up