Overview

  • Product nameAnti-Vitellogenin antibody
    See all Vitellogenin primary antibodies
  • Description
    Rabbit polyclonal to Vitellogenin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    PO-2 binds to vitellogenin in plasma samples from sea bream. The antibody cross-reacts well with Vtg in plasma samples from Atlantic salmon (Salmo salar), brown trout (Salmo trutta), rainbow trout (Oncorhynchus mykiss), turbot (Scophthalmus maximus), flounder (Platichthys flesus), Atlantic cod (Gadus morhua), wrasse (Ctenolabrus rupestris), tilapia (Oreochromis niloticus) and carp ( Cyprinus carpio). The degree of cross-reactivity differ between species and with the different methods employed.
  • Immunogen

    Vitellogenin purified from plasma of 17ß-estradiol treated sea bream.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 0.1M Sodium phosphate. pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/200 - 1/3000.
    WB: 1/200 - 1/3000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • Cellular localizationSecreted
    • Alternative names
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Anti-Vitellogenin antibody images

    • Coating: Plasma (diluted 1:1000) from 17 beta-estradiol treated (E2) or control (C) fish. Primary antibody: PO-2
    • All lanes : Anti-Vitellogenin antibody (ab36877) at 1/3000 dilution

      Lane 1 : MW standard
      Lane 2 : Wrasse (E2)
      Lane 3 : Wrasse (C)
      Lane 4 : Tilapia (E2)
      Lane 5 : Tilapia (C)
      Lane 6 : Carp (E2)
      Lane 7 : Carp (C)

    References for Anti-Vitellogenin antibody (ab36877)

    ab36877 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"