Anti-Vitellogenin antibody (ab36881)

Overview

  • Product nameAnti-Vitellogenin antibody
    See all Vitellogenin primary antibodies
  • Description
    Rabbit polyclonal to Vitellogenin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with Arctic char. It cross reacts with Atlantic salmon (Salmo salar), brown trout (Salmo trutta), Atlantic cod (Gadus morhua), turbot (Scophthalmus maximus), flounder (Platichthys flesus), wrasse (Ctenolabrus rupestris) and tilapia (Oreochromis niloticus), and zebrafish (Danio rerio). Not yet tested in other species.
  • Immunogen

    Vitellogenin purified from plasma of 17 beta estradiol treated Arctic char.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate. pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36881 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1:1000 - 1:3000 (Salmonids)
    1:500 - 1:1000 (Other species)

    WB: 1:1000 - 1:3000 (Salmonids)
    1:500 - 1:1000 (Other species) . Predicted molecular weight: 183 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • Cellular localizationSecreted
    • Alternative names
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Anti-Vitellogenin antibody images

    • Coating: Plasma (diluted 1:1000) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish. Primary antibody: PO-1
    • All lanes : Anti-Vitellogenin antibody (ab36881) at 1/3000 dilution

      Lane 1 : MW standard
      Lane 2 : Salmon (E2)
      Lane 3 : Salmon (C)
      Lane 4 : Cod (E2)
      Lane 5 : Cod (C)
      Lane 6 : Carp (E2)
      Lane 7 : Carp (C)


      Predicted band size : 183 kDa

    References for Anti-Vitellogenin antibody (ab36881)

    ab36881 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"