Anti-Vitellogenin antibody [BN-5] (ab36797)

Overview

  • Product name
    Anti-Vitellogenin antibody [BN-5]
    See all Vitellogenin primary antibodies
  • Description
    Mouse monoclonal [BN-5] to Vitellogenin
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rainbow trout, Salmo salar
  • Immunogen

    Full length native vitellogenin purified from plasma of 17 beta estradiol induced Atlantic salmon (Salmo salar).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Clonality
    Monoclonal
  • Clone number
    BN-5
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 180 kDa.
ELISA 1/100 - 1/2000.

Target

  • Relevance
    Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
  • Cellular localization
    Secreted
  • Database links
    • Alternative names
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Anti-Vitellogenin antibody [BN-5] images

    • Coating: Plasma (diluted 1:1000) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish. Primary antibody: BN-5.
    • All lanes : Anti-Vitellogenin antibody [BN-5] (ab36797) at 1/100 dilution

      Lane 1 : MW Standard
      Lane 2 : Salmon (C)
      Lane 3 : Salmon (E2)
      Lane 4 : Brown trout (C)
      Lane 5 : Brown trout (E)
      Lane 6 : Rainbow trout (C)
      Lane 7 : Rainbow trout (E2)


      Predicted band size : 180 kDa

    References for Anti-Vitellogenin antibody [BN-5] (ab36797)

    ab36797 has not yet been referenced specifically in any publications.

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    Thank you for contacting us and your interest in our products.
    We do have an antibody which has been raised against full length native vitellogenin purified from plasma of 17 beta estradiol induced Atlantic Salmon (Salmo salar) which may be suitab...

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