Anti-Vitellogenin antibody [BN-5] (ab36797)
Key features and details
- Mouse monoclonal [BN-5] to Vitellogenin
- Suitable for: WB, ELISA
- Reacts with: Fish, Rainbow trout, Salmo salar, Salvelinus alpinus
- Isotype: IgG1
Overview
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Product name
Anti-Vitellogenin antibody [BN-5]
See all Vitellogenin primary antibodies -
Description
Mouse monoclonal [BN-5] to Vitellogenin -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Fish, Rainbow trout, Salmo salar, Brown trout, Salvelinus alpinus -
Immunogen
Full length native vitellogenin purified from plasma of 17 beta estradiol induced Atlantic salmon (Salmo salar).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
BN-5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36797 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 180 kDa.
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ELISA |
1/100 - 1/2000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 180 kDa. |
ELISA
1/100 - 1/2000. |
Target
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Relevance
Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs). -
Cellular localization
Secreted -
Database links
- SwissProt: Q91062 Fish
- SwissProt: Q92093 Rainbow trout
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Alternative names
- Vg antibody
- VTG antibody
- VTG1 antibody
Images
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Coating: Plasma (diluted 1:1000) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish. Primary antibody: BN-5.
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All lanes : Anti-Vitellogenin antibody [BN-5] (ab36797) at 1/100 dilution
Lane 1 : MW Standard
Lane 2 : Salmon (C)
Lane 3 : Salmon (E2)
Lane 4 : Brown trout (C)
Lane 5 : Brown trout (E)
Lane 6 : Rainbow trout (C)
Lane 7 : Rainbow trout (E2)
Predicted band size: 180 kDa
Samples: Plasma (1:200, 7 µl/well) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab36797 has been referenced in 1 publication.
- Zheng R et al. Joint effects of chronic exposure to environmentally relevant levels of nonylphenol and cadmium on the reproductive functions in male rockfish Sebastiscus marmoratus. Comp Biochem Physiol C Toxicol Pharmacol 215:25-32 (2019). PubMed: 30315922