Anti-Vitellogenin antibody [BN-5] (ab36797)

Overview

  • Product nameAnti-Vitellogenin antibody [BN-5]
    See all Vitellogenin primary antibodies
  • Description
    Mouse monoclonal [BN-5] to Vitellogenin
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Rainbow Trout, Salmo salar
  • Immunogen

    Full length native vitellogenin purified from plasma of 17 beta estradiol induced Atlantic salmon (Salmo salar).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberBN-5
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36797 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 180 kDa.
ELISA 1/100 - 1/2000.

Target

  • RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
  • Cellular localizationSecreted
  • Database links
    • Alternative names
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Anti-Vitellogenin antibody [BN-5] images

    • Coating: Plasma (diluted 1:1000) from 17 beta-estradiol treated (E2), sexually mature female (E) or control (C) fish. Primary antibody: BN-5.
    • All lanes : Anti-Vitellogenin antibody [BN-5] (ab36797) at 1/100 dilution

      Lane 1 : MW Standard
      Lane 2 : Salmon (C)
      Lane 3 : Salmon (E2)
      Lane 4 : Brown trout (C)
      Lane 5 : Brown trout (E)
      Lane 6 : Rainbow trout (C)
      Lane 7 : Rainbow trout (E2)


      Predicted band size : 180 kDa

    References for Anti-Vitellogenin antibody [BN-5] (ab36797)

    ab36797 has not yet been referenced specifically in any publications.

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