Anti-Vitellogenin antibody [JE-10D4] (ab36804)
Key features and details
- Mouse monoclonal [JE-10D4] to Vitellogenin
- Suitable for: ELISA, WB
- Reacts with: Carp, Zebrafish
- Isotype: IgG1
Overview
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Product name
Anti-Vitellogenin antibody [JE-10D4]
See all Vitellogenin primary antibodies -
Description
Mouse monoclonal [JE-10D4] to Vitellogenin -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Carp, Zebrafish, Brown bullhead, Common roach, Fathead minnow -
Immunogen
Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.08% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JE-10D4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/100 - 1/1000.
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WB |
1/100 - 1/1000.
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Notes |
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ELISA
1/100 - 1/1000. |
WB
1/100 - 1/1000. |
Target
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Relevance
Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs). -
Cellular localization
Secreted -
Alternative names
- Vg antibody
- VTG antibody
- VTG1 antibody
Images
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All lanes : Anti-Vitellogenin antibody [JE-10D4] (ab36804) at 1/500 dilution
Lane 2 : Zebrafish purified vitellogenin at 1 µg
Lane 3 : Zebrafish whole body homogenate (17B-estradiol treated) at 10 µg
Lane 4 : Zebrafish whole body homogenate (control) at 10 µg
Lane 5 : Carp plasma (17B-estradiol treated) at 10 µl
Lane 6 : Carp plasma (control) at 10 µl
Lane 7 : Fathead minno plasma (17B-estradiol treated) at 10 µl
Lane 8 : Fathead minno plasma (control) at 10 µg
Lane 9 : Roach plasma (control) at 10 µg -
ELISA using ab36804 for measurement of vitellogenin in various samples.
ELISA coating: Whole body homogenate (wbh; 10 µg protein/ml) or plasma (pl; diluted 1:1000) from 17β-estradiol treated (E2) or control (C) fsh. Purifed vitellogenin (Vtg; 2 µg/ml).
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab36804 has been referenced in 3 publications.
- Cozza G et al. The Golgi 'casein kinase' Fam20C is a genuine 'phosvitin kinase' and phosphorylates polyserine stretches devoid of the canonical consensus. FEBS J 285:4674-4683 (2018). PubMed: 30387551
- Li Q et al. FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis. Biosci Rep 35:N/A (2015). PubMed: 26430246
- Fan X et al. Selective yolk deposition and mannose phosphorylation of lysosomal glycosidases in zebrafish. J Biol Chem 285:32946-53 (2010). WB ; Zebrafish . PubMed: 20729204