Overview

  • Product nameAnti-Vitellogenin antibody [JE-10D4]
    See all Vitellogenin primary antibodies
  • Description
    Mouse monoclonal [JE-10D4] to Vitellogenin
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Zebrafish
  • Immunogen

    Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 12.3mM Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberJE-10D4
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/100 - 1/1000.
    Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
    WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • Cellular localizationSecreted
    • Alternative names
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Anti-Vitellogenin antibody [JE-10D4] images



    • Predicted band size : 183 kDa

      Image from Fan X et al, J Biol Chem. 2010 Oct 22;285(43):32946-53. Epub 2010 Aug 20, Fig 6. DOI 10.1074/jbc.M110.158295

    References for Anti-Vitellogenin antibody [JE-10D4] (ab36804)

    This product has been referenced in:
    • Fan X  et al. Selective yolk deposition and mannose phosphorylation of lysosomal glycosidases in zebrafish. J Biol Chem 285:32946-53 (2010). WB ; Zebrafish . Read more (PubMed: 20729204) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"