The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/100 - 1/1000.
Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
Western blot - Vitellogenin antibody [JE-10D4] (ab36804)Image from Fan X et al, J Biol Chem. 2010 Oct 22;285(43):32946-53. Epub 2010 Aug 20, Fig 6. DOI 10.1074/jbc.M110.158295
Zebrafish yolk lysates (6 hours post fertilization) were resolved by 10% SDS-PAGE, transferred to nitrocellulose, and probed with ab36804 at a 1/1000 dilution. The blot was then incubated with streptavidin-linked HRP and bands were detected with standard ECL reagents.