Anti-Vitellogenin antibody [JE-2A6] (ab36805)
Key features and details
- Mouse monoclonal [JE-2A6] to Vitellogenin
- Suitable for: WB
- Reacts with: Zebrafish
- Isotype: IgG2b
Overview
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Product name
Anti-Vitellogenin antibody [JE-2A6]
See all Vitellogenin primary antibodies -
Description
Mouse monoclonal [JE-2A6] to Vitellogenin -
Host species
Mouse -
Specificity
Does not cross-react with vitellogenin from the other cyrinid species carp (Cyprinus carpio), fathead minnow (Pimephales promelas) or roach (Rutilus rutilus). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.08% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
JE-2A6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab36805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Predicted molecular weight: 183 kDa.
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Notes |
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WB
1/100 - 1/1000. Predicted molecular weight: 183 kDa. |
Target
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Relevance
Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs). -
Cellular localization
Secreted -
Alternative names
- Vg antibody
- VTG antibody
- VTG1 antibody
Images
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All lanes : Anti-Vitellogenin antibody [JE-2A6] (ab36805) at 1/200 dilution
Lane 1 : Purified zebrafish vitellogenin at 2 µg
Lane 2 : Zebrafish whole body homogenate, 17ß-estradiol treated at 10 µg
Lane 3 : Zebrafish whole body homogenate at 10 µg
Lane 4 : Carp plasma, 17ß-estradiol treated at 10 µl
Lane 5 : Carp plasma at 10 µl
Lane 6 : Fathead minnow plasma, 17ß-estradiol treated at 10 µl
Lane 7 : Fathead minnow plasma at 10 µl
Lane 8 : Roach plasma, 17ß-estradiol treated at 10 µl
Lane 9 : Roach plasma at 10 µl
Predicted band size: 183 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab36805 has not yet been referenced specifically in any publications.