Anti-Vitellogenin antibody [JE-2A6] (ab36805)

Overview

  • Product nameAnti-Vitellogenin antibody [JE-2A6]
    See all Vitellogenin primary antibodies
  • Description
    Mouse monoclonal [JE-2A6] to Vitellogenin
  • SpecificityDoes not cross-react with vitellogenin from the other cyrinid species carp (Cyprinus carpio), fathead minnow (Pimephales promelas) or roach (Rutilus rutilus).
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Zebrafish
  • Immunogen

    Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 12.3mM Sodium Azide
    Constituents: 1% BSA, 0.15M Sodium chloride, 0.1M Sodium phosphate, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberJE-2A6
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notesELISA: 1/100 - 1/1000.
    Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
    WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • Cellular localizationSecreted
    • Alternative names
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Anti-Vitellogenin antibody [JE-2A6] images

    • All lanes : Anti-Vitellogenin antibody [JE-2A6] (ab36805) at 1/200 dilution

      Lane 1 : Purified zebrafish vitellogenin at 2 µg
      Lane 2 : Zebrafish whole body homogenate, 17ß-estradiol treated at 10 µg
      Lane 3 : Zebrafish whole body homogenate at 10 µg
      Lane 4 : Carp plasma, 17ß-estradiol treated at 10 µl
      Lane 5 : Carp plasma at 10 µl
      Lane 6 : Fathead minnow plasma, 17ß-estradiol treated at 10 µl
      Lane 7 : Fathead minnow plasma at 10 µl
      Lane 8 : Roach plasma, 17ß-estradiol treated at 10 µl
      Lane 9 : Roach plasma at 10 µl


      Predicted band size : 183 kDa

    References for Anti-Vitellogenin antibody [JE-2A6] (ab36805)

    ab36805 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"