Anti-Vitellogenin antibody [ND-3G6] (ab36794)

Overview

  • Product nameAnti-Vitellogenin antibody [ND-3G6]
    See all Vitellogenin primary antibodies
  • Description
    Mouse monoclonal [ND-3G6] to Vitellogenin
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    The monoclonal antibody ND-3G6 binds with high affinity to vitellogenin from Japanese quail (coturnix japonica), common tern (Sterna hirundo) and barn swallow (Hirundo rustica). The antibody also cross-reacts with vitellogenin from other bird species, including American kestrel (Falco sparverius), zebra finch (Taeniopygia guttata) and domestic chicken (Gallus gallus). The degree of cross-reactivity differs between species and with the methods employed.
  • Immunogen

    Mixture of Vitellogenin from Japanese quail (coturnix japonica), common tern (Sterna hirundo) and barn swallow (Hirundo rustica).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: BSA
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberND-3G6
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ELISA
WB
  • Application notesELISA: 1/100 - 1/1000.
    WB: 1/50 - 1/500.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • Cellular localizationSecreted
    • Alternative names
      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Anti-Vitellogenin antibody [ND-3G6] images

    • Coating: Purified Vtg (2 μg/ml) or egg yolk (y; diluted 1:500). Primary antibody: ND-3G6
    • ab36794 staining Vitellogenin in chicken ovary sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Tissue was fixed in formaldehyde, blocked using 1% BSA for 30 minutes at 20°C, then incubated with ab36794 at a 1/20 dilution for 20 hours at 4°C. The secondary used was a 5nm Gold conjugated goat anti-rabbit polyclonal used at a 1/40 dilution. Counterstained with HandE.

      See Abreview

    References for Anti-Vitellogenin antibody [ND-3G6] (ab36794)

    ab36794 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Chicken Tissue sections (ovaries)
    Specification ovaries
    Fixative Formaldehyde
    Antigen retrieval step None
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C
    Username

    Dr. Peter Wooding

    Verified customer

    Submitted Sep 20 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"