Overview

  • Product nameAnti-Vitronectin antibody [A18]
    See all Vitronectin primary antibodies
  • Description
    Mouse monoclonal [A18] to Vitronectin
  • Specificityab23444 is highly specific for Vitronectin. There is no evidence for cross reactivity with other connective tissue proteins (Fibronectin, Elastin, Collagen, Laminin).
  • Tested applicationsSuitable for: IHC-P, Inhibition Assay, ELISA, IHC-Frmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Rabbit, Horse, Cow
    Does not react with: Sheep, Human
  • Immunogen

    Tissue/ cell preparation (Cow): Lysed bovine corneal endothelial cells and extracellular matrix

  • General notes

    ab23444 can be used to probe vitronectin conformation.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 500mM Sodium chloride, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberA18
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.

ab23444 inhibits integrin mediated cell adhesion to bovine vitronectin.

ELISA 1/500.
IHC-Fr 1/500.

1/500 for PLP fixed samples.

  • Application notesIs unsuitable for WB.
  • Target

    • FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
      Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
    • Tissue specificityPlasma.
    • Sequence similaritiesContains 4 hemopexin repeats.
      Contains 1 SMB (somatomedin-B) domain.
    • DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
    • Post-translational
      modifications
      Sulfated on 2 tyrosine residues.
      N- and O-glycosylated.
      Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
      Phosphorylation sites are present in the extracellular medium.
    • Cellular localizationSecreted, extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all

    References for Anti-Vitronectin antibody [A18] (ab23444)

    ab23444 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab23444.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"