Overview

  • Product nameAnti-Vitronectin antibody [BV1]
    See all Vitronectin primary antibodies
  • Description
    Mouse monoclonal [BV1] to Vitronectin
  • SpecificityThe antibody binds to soluble vitronectin as well as to membrane bound vitronectin.
  • Tested applicationsSuitable for: IHC-P, WB, IP, ICC/IF, Purificationmore details
  • Species reactivity
    Reacts with: Human

Properties

Applications

Our Abpromise guarantee covers the use of ab7165 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. PubMed: 19730772
WB Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Purification Use at an assay dependent concentration.

Target

  • FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificityPlasma.
  • Sequence similaritiesContains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications
    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted, extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

References for Anti-Vitronectin antibody [BV1] (ab7165)

This product has been referenced in:
  • Knappe UJ  et al. Expression of extracellular matrix-proteins in perisellar connective tissue and dura mater. Acta Neurochir (Wien) 152:345-53 (2010). IHC-P ; Human . Read more (PubMed: 19730772) »

See 1 Publication for this product

Product Wall

We regret to hear about your problems with ab7165 monoclonal antibody to human vitronectin. The antibody is described in the following article: Zanetti, A et al; Clustering of vitronectin and RGD peptides on microspheres leads to engagement of inte...

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I have contacted the source of the antibody to find out if they can recommend a protocol. Could you please confirm you used protease inhibitors and kept the samples cold at all times during your experiment prior to boiling your samples with loading...

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I'm sorry you are having problems with ab7165 and would like to thank you for taking the time to give me details of your protocol, it is very useful. I would like to suggest incubating overnight at 4C and try also more concentrated antibody. Co...

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The subunit recognise by this antibody is undetermined, as is it's cross-reactivity with mouse.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"