Anti-Vitronectin antibody [EP781Y] (ab46808)

Overview

  • Product nameAnti-Vitronectin antibody [EP781Y]
    See all Vitronectin primary antibodies
  • Description
    Rabbit monoclonal [EP781Y] to Vitronectin
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues near the hemopexin-like 1 domain of human Vitronectin.

  • Positive control
    • Human serum; human lung tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • Purification notesThis antibody has not been purified. It is provided in tissue culture supernatant and storage buffer.
  • ClonalityMonoclonal
  • Clone numberEP781Y
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab46808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 54,60,75 kDa (predicted molecular weight: 54 kDa).
IP 1/50.
IHC-P 1/100 - 1/250.

Target

  • FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificityPlasma.
  • Sequence similaritiesContains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications
    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted, extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

Anti-Vitronectin antibody [EP781Y] images

  • Anti-Vitronectin antibody [EP781Y] (ab46808) at 1/20000 dilution + 10ug Human serum

    Secondary
    Goat anti-rabbit, HRP labelled. at 1/2000 dilution

    Predicted band size : 54 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 54 kDa (possible non-specific binding),60 kDa (possible non-specific binding).
  • This image shows human lung tissue treated with ab46808 at 1/100 dilution.

References for Anti-Vitronectin antibody [EP781Y] (ab46808)

ab46808 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"