Overview

  • Product nameAnti-Vitronectin antibody [EP873Y]
    See all Vitronectin primary antibodies
  • Description
    Rabbit monoclonal [EP873Y] to Vitronectin
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the V10 subunit of human Vitronectin.

  • Positive control
    • Human liver tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-Vitronectin antibody (HRP) [EP873Y] (ab199310)

Properties

Applications

Our Abpromise guarantee covers the use of ab45139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 75 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
      Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
    • Tissue specificityPlasma.
    • Sequence similaritiesContains 4 hemopexin repeats.
      Contains 1 SMB (somatomedin-B) domain.
    • DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
    • Post-translational
      modifications
      Sulfated on 2 tyrosine residues.
      N- and O-glycosylated.
      Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
      Phosphorylation sites are present in the extracellular medium.
    • Cellular localizationSecreted, extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all

    Anti-Vitronectin antibody [EP873Y] images

    • Anti-Vitronectin antibody [EP873Y] (ab45139) at 1/20000 dilution + Hu Serum membrane at 10 µg

      Secondary
      Goat anti-rabbit HRP labelled, at 1/2000 dilution

      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Ab45139 at a 1/100 dilution, staining human Vitronectin in human liver tissue by immunohistochemistry using paraffin embedded tissue.

    References for Anti-Vitronectin antibody [EP873Y] (ab45139)

    This product has been referenced in:
    • Kapinas K  et al. Bone matrix osteonectin limits prostate cancer cell growth and survival. Matrix Biol 31:299-307 (2012). WB . Read more (PubMed: 22525512) »
    • Tamay AG  et al. Evaluation of the effects of gonadotropin-releasing hormone antagonist (GnRH-ant) and agonist (GnRH-a) in the prevention of postoperative adhesion formation in a rat model with immunohistochemical analysis. Fertil Steril 96:1230-3 (2011). Read more (PubMed: 21963228) »

    See all 3 Publications for this product

    Product Wall

    Please find attached to this email the MSDS you requested for the following antibodies:
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