Anti-Vitronectin antibody [EP873Y] (ab45139)


  • Product name
    Anti-Vitronectin antibody [EP873Y]
    See all Vitronectin primary antibodies
  • Description
    Rabbit monoclonal [EP873Y] to Vitronectin
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the V10 subunit of human Vitronectin.

  • Positive control
    • Human liver tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab45139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 75 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
      Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
    • Tissue specificity
    • Sequence similarities
      Contains 4 hemopexin repeats.
      Contains 1 SMB (somatomedin-B) domain.
    • Domain
      The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
    • Post-translational
      Sulfated on 2 tyrosine residues.
      N- and O-glycosylated.
      Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
      Phosphorylation sites are present in the extracellular medium.
    • Cellular localization
      Secreted, extracellular space.
    • Information by UniProt
    • Database links
    • Alternative names
      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all


    • Anti-Vitronectin antibody [EP873Y] (ab45139) at 1/20000 dilution + Hu Serum membrane at 10 µg

      Goat anti-rabbit HRP labelled, at 1/2000 dilution

      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • Ab45139 at a 1/100 dilution, staining human Vitronectin in human liver tissue by immunohistochemistry using paraffin embedded tissue.


    This product has been referenced in:
    • Piccolo SR  et al. Integrative analyses reveal signaling pathways underlying familial breast cancer susceptibility. Mol Syst Biol 12:860 (2016). WB ; Human . Read more (PubMed: 26969729) »
    • Salazar-Peláez LM  et al. Vitronectin expression in the airways of subjects with asthma and chronic obstructive pulmonary disease. PLoS One 10:e0119717 (2015). Read more (PubMed: 25768308) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Please find attached to this email the MSDS you requested for the following antibodies:

    A link to each of those MSDS will be shortly available from the product datasheets. This usually takes a few...

    Read More

    Thank you for contacting us.

    I would like to inform you that we are currently dealing with your request.

    I will contact you again as soon as the requested documents are available.

    Thank you for your patience.

    Thank you for contacting Abcam.

    According to the details of our testing program we "offer a discount equal to one free antibody of your choice off a future purchase if you buy ab45139 now, test it in IHC-Fr and submit feedback to us in ...

    Read More

    DISCOUNT CODE: ***********
    Expiration date: ************

    I am very pleased to hear you would like to accept our offer and test ab45139 in IHC-Frozen tissue.This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To r...

    Read More

    Thank you very much for your interest in ab45139.

    To our knowledge, ab45139 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab45139 now, test it in IHC-Fr and submit feedback to us in the for...

    Read More

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab62769.

    To check the status of the order please contact o...

    Read More


    Sign up