Anti-Vitronectin antibody [VN58-1] (ab13413)

Overview

  • Product nameAnti-Vitronectin antibody [VN58-1]
    See all Vitronectin primary antibodies
  • Description
    Mouse monoclonal [VN58-1] to Vitronectin
  • SpecificityThis antibody does not interfere with vitronectin-mediated adhesion.
  • Tested applicationsSuitable for: ICC/IF, ELISA, WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Cow
  • Immunogen

    Human Vitronectin.

  • EpitopeThis antibody specifically reacts with an epitope in the region of amino acids 1-130 of human vitronectin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, 10mM PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberVN58-1
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/3000. (with solid phase antigen)
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificityPlasma.
  • Sequence similaritiesContains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications
    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localizationSecreted, extracellular space.
  • Information by UniProt
  • Database links
  • Alternative names
    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

Anti-Vitronectin antibody [VN58-1] images

  • This picture shows formalin-fixed paraffin embedded human skin stained with Vitronectin (1:100 - 30 minutes RT). The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
  • ICC/IF image of ab13413 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13413, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Vitronectin antibody [VN58-1] (ab13413)

This product has been referenced in:
  • Zhou J  et al. Direct role of interrod spacing in mediating cell adhesion on Sr-HA nanorod-patterned coatings. Int J Nanomedicine 9:1243-60 (2014). ICC/IF ; Human . Read more (PubMed: 24634585) »
  • Stepanek O  et al. Interaction of late apoptotic and necrotic cells with vitronectin. PLoS One 6:e19243 (2011). Read more (PubMed: 21573223) »

See all 5 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 100 µg
Treatment Perifosine 24 hr
Specification Epithelial OVCA cell line
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Aug 12 2016

Thank you for getting back to me with those details. Having reviewed your protocol there are a few things I may be able to suggest. The dilution for ab13413 is recommended in the range of 5-10µg/ml and ab27710 at an assay dependent dilution. I ...

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Thank you for contacting us. I am sorry that you are experiencing problems with these antibodies. In order to assist you as best I can could you please fill in the questionnaire I have attached to this email. This will allow me to understand more ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"