Overview

  • Product nameAnti-VKORC1 antibody
    See all VKORC1 primary antibodies
  • Description
    Rabbit polyclonal to VKORC1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide corresponding to a region between N terminal amino acids 22-49 of Human VKORC1 (NP_076869.1).

  • Positive control
    • NCI-H460 cell line lysates

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab108053 is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionInvolved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K.
  • Tissue specificityExpressed at highest levels in fetal and adult liver, followed by fetal heart, kidney, and lung, adult heart, and pancreas.
  • Involvement in diseaseDefects in VKORC1 are a cause of combined deficiency of vitamin K-dependent clotting factors type 2 (VKCFD2) [MIM:607473]. VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K.
    Defects in VKORC1 are a cause of coumarin resistance (CMRES) [MIM:122700]; also known as warfarin resistance. Warfarin and other coumarin-type anticoagulants are used to reduce blood viscosity in the treatment of thromboembolic disorders.
  • Sequence similaritiesBelongs to the VKOR family.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-epoxide reductase subunit 1 antibody
    • EDTP308 antibody
    • FLJ00289 antibody
    • IMAGE3455200 antibody
    • MGC2694 antibody
    • MST134 antibody
    • MST576 antibody
    • Phylloquinone epoxide reductase antibody
    • Vitamin K dependent clotting factors deficiency 2 antibody
    • Vitamin K epoxide reductase complex subunit 1 antibody
    • Vitamin K1 2 antibody
    • Vitamin K1 2,3-epoxide reductase subunit 1 antibody
    • Vitamin K1 epoxide reductase (warfarin sensitive) antibody
    • VKCFD2 antibody
    • VKOR antibody
    • VKOR1_HUMAN antibody
    • VKORC1 antibody
    see all

Anti-VKORC1 antibody images

  • Anti-VKORC1 antibody (ab108053) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 18 kDa
  • ab108053, at a 1/50 dilution, staining VKORC1 in formalin fixed and paraffin embedded Human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

References for Anti-VKORC1 antibody (ab108053)

ab108053 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Primary hepatocyte culture)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Specification Primary hepatocyte culture
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2015

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (12% Bis-Tris)
Sample Human Cell lysate - whole cell (HEK293, Y79, HepG2, A549)
Specification HEK293, Y79, HepG2, A549
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"