Overview

  • Product name
  • Description
    Rabbit polyclonal to VKORC1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide corresponding to a region between N terminal amino acids 22-49 of Human VKORC1 (NP_076869.1).

  • Positive control
    • NCI-H460 cell line lysates

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab108053 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 18 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Involved in vitamin K metabolism. Catalytic subunit of the vitamin K epoxide reductase (VKOR) complex which reduces inactive vitamin K 2,3-epoxide to active vitamin K.
  • Tissue specificity
    Expressed at highest levels in fetal and adult liver, followed by fetal heart, kidney, and lung, adult heart, and pancreas.
  • Involvement in disease
    Defects in VKORC1 are a cause of combined deficiency of vitamin K-dependent clotting factors type 2 (VKCFD2) [MIM:607473]. VKCFD leads to a bleeding tendency that is usually reversed by oral administration of vitamin K.
    Defects in VKORC1 are a cause of coumarin resistance (CMRES) [MIM:122700]; also known as warfarin resistance. Warfarin and other coumarin-type anticoagulants are used to reduce blood viscosity in the treatment of thromboembolic disorders.
  • Sequence similarities
    Belongs to the VKOR family.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-epoxide reductase subunit 1 antibody
    • EDTP308 antibody
    • FLJ00289 antibody
    • IMAGE3455200 antibody
    • MGC2694 antibody
    • MST134 antibody
    • MST576 antibody
    • Phylloquinone epoxide reductase antibody
    • Vitamin K dependent clotting factors deficiency 2 antibody
    • Vitamin K epoxide reductase complex subunit 1 antibody
    • Vitamin K1 2 antibody
    • Vitamin K1 2,3-epoxide reductase subunit 1 antibody
    • Vitamin K1 epoxide reductase (warfarin sensitive) antibody
    • VKCFD2 antibody
    • VKOR antibody
    • VKOR1_HUMAN antibody
    • VKORC1 antibody
    see all

Images

  • Anti-VKORC1 antibody (ab108053) at 1/100 dilution + NCI-H460 cell line lysates at 35 µg

    Predicted band size : 18 kDa
  • ab108053, at a 1/50 dilution, staining VKORC1 in formalin fixed and paraffin embedded Human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab108053 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (Primary hepatocyte culture)
Gel Running Conditions
Reduced Denaturing
Loading amount
25 µg
Specification
Primary hepatocyte culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12% Bis-Tris)
Sample
Human Cell lysate - whole cell (HEK293, Y79, HepG2, A549)
Specification
HEK293, Y79, HepG2, A549
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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