Overview

  • Product nameAnti-VLDL Receptor antibody [1H10]
    See all VLDL Receptor primary antibodies
  • Description
    Mouse monoclonal [1H10] to VLDL Receptor
  • Tested applicationsSuitable for: WB, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the C-terminal region of Human VLDL Receptor

  • General notes


    Blocks apoE4 binding.

Properties

Applications

Our Abpromise guarantee covers the use of ab75591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Use under non reducing condition. Predicted molecular weight: 96 kDa.
Flow Cyt Use 2µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.

Target

  • FunctionBinds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.
  • Tissue specificityAbundant in heart and skeletal muscle; also ovary and kidney; not in liver.
  • Involvement in diseaseDefects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1) [MIM:224050]; also known as dysequilibrium syndrome (DES) or non-progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures.
  • Sequence similaritiesContains 3 EGF-like domains.
    Contains 8 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • Post-translational
    modifications
    Ubiquitinated at Lys-839 by MYLIP leading to degradation.
  • Cellular localizationMembrane. Membrane > clathrin-coated pit.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35024 antibody
    • Very low density lipoprotein receptor antibody
    • Very low-density lipoprotein receptor antibody
    • VLDL R antibody
    • VLDL receptor antibody
    • VLDL-R antibody
    • VLDLR antibody
    • VLDLR_HUMAN antibody
    • VLDLRCH antibody
    see all

Anti-VLDL Receptor antibody [1H10] images

  • Overlay histogram showing HEK293 cells stained with ab75591 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75591, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Flow cytometry analysis of Human THP-1 cell lines, staining VLDL Receptor (red histogram) with ab75591.

    Cells were taken from suspension culture and washed with PBS/BSA before fixation. Cells were blocked and permeabilized in 10% donkey serum/0.3 M glycine/0.1% BSA/0.1% Tween-20 in PBS for 20 mins at room temperature. The sample was incubated with the primary antibody (2 µg/106 cells in 0.1% BSA/0.1% Tween-20 in PBS) for 30 minutes at 22°C. A FITC-conjugated donkey anti-mouse polyclonal IgG (30 µg/ml) was used as the secondary antibody.

    Gating Strategy: FSC/SSC

    See Abreview

References for Anti-VLDL Receptor antibody [1H10] (ab75591)

This product has been referenced in:
  • Gardner JK  et al. Lipid-laden partially-activated plasmacytoid and CD4(-)CD8a(+) dendritic cells accumulate in tissues in elderly mice. Immun Ageing 11:11 (2014). Flow Cyt ; Mouse . Read more (PubMed: 25089147) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (brain and embryo)
Loading amount 20 µg
Specification brain and embryo
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Fusun Balli

Verified customer

Submitted Jan 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (THP-1 cell line)
Specification THP-1 cell line
Preparation Cell harvesting/tissue preparation method: Cells were taken from suspension culture and washed with PBS/BSA before fixation
Sample buffer: 0.1% BSA in PBS
Fixation 80% Methanol
Permeabilization Yes - 0.1% Tween 20
Gating Strategy Cell population was gated by FSC and SSC
Username

Abcam user community

Verified customer

Submitted Oct 26 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (non-small lung carcinoma cells)
Loading amount 25 µg
Specification non-small lung carcinoma cells
Gel Running Conditions Non-reduced Denaturing (10%)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Jul 02 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"