Anti-VLDL Receptor/VLDL-R antibody (ab203271)
Key features and details
- Rabbit polyclonal to VLDL Receptor/VLDL-R
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-VLDL Receptor/VLDL-R antibody
See all VLDL Receptor/VLDL-R primary antibodies -
Description
Rabbit polyclonal to VLDL Receptor/VLDL-R -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human VLDL Receptor/VLDL-R aa 550-650 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P98155 -
Positive control
- WB: Siha, A549, 293T and A421 cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Predicted molecular weight: 96 kDa.
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Notes |
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WB
1/100 - 1/1000. Predicted molecular weight: 96 kDa. |
Target
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Function
Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation. -
Tissue specificity
Abundant in heart and skeletal muscle; also ovary and kidney; not in liver. -
Involvement in disease
Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1) [MIM:224050]; also known as dysequilibrium syndrome (DES) or non-progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures. -
Sequence similarities
Contains 3 EGF-like domains.
Contains 8 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
Post-translational
modificationsUbiquitinated at Lys-839 by MYLIP leading to degradation. -
Cellular localization
Membrane. Membrane > clathrin-coated pit. - Information by UniProt
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Database links
- Entrez Gene: 7436 Human
- Omim: 192977 Human
- SwissProt: P98155 Human
- Unigene: 370422 Human
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Alternative names
- FLJ35024 antibody
- Very low density lipoprotein receptor antibody
- Very low-density lipoprotein receptor antibody
see all
Images
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All lanes : Anti-VLDL Receptor/VLDL-R antibody (ab203271) at 1/1000 dilution
Lane 1 : Human A549 cell lysates
Lane 2 : Human 293T cell lysates
Lane 3 : Human A431 cell lysates
Lane 4 : Human Siha cell lysates
Secondary
All lanes : A conjugated secondary antibody incubation at 1/20000 dilution
Predicted band size: 96 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab203271 has been referenced in 3 publications.
- Imai T et al. Different types of reactions to E7386 among colorectal cancer patient-derived organoids and corresponding CAFs. Oncol Lett 24:221 (2022). PubMed: 35707761
- Lin YS et al. Electronegative very-low-density lipoprotein induces brain inflammation and cognitive dysfunction in mice. Sci Rep 11:6013 (2021). PubMed: 33727609
- Patel M et al. Osteopontin and LDLR Are Upregulated in Hearts of Sudden Cardiac Death Victims With Heart Failure With Preserved Ejection Fraction and Diabetes Mellitus. Front Cardiovasc Med 7:610282 (2020). PubMed: 33330671