Overview

  • Product name
    Anti-VMA2 antibody [13D11B2]
  • Description
    Mouse monoclonal [13D11B2] to VMA2
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Full length native protein (purified)

  • Positive control
    • Yeast membranes
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab113684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

Target

  • Function
    Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. It is an electrogenic proton pump that generates a proton motive force of 180 mv, inside positive and acidic, in the vacuolar membrane vesicles.
  • Sequence similarities
    Belongs to the ATPase alpha/beta chains family.
  • Information by UniProt
  • Database links
    • Alternative names
      • V-ATPase 57 kDa subunit antibody
      • V-ATPase subunit B antibody
      • V-type proton ATPase subunit B antibody
      • Vacuolar proton pump subunit B antibody
      • VATB_YEAST antibody
      • VMA2 antibody
      see all

    Images

    • Anti-VMA2 antibody [13D11B2] (ab113684) + Yeast whole cell lysate at 15 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 58 kDa

    References

    This product has been referenced in:
    • Belgareh-Touzé N  et al. Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy. Autophagy 13:114-132 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 27846375) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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