Overview

  • Product nameAnti-VMA21 antibody
  • Description
    Rabbit polyclonal to VMA21
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: MERPDKAALN ALQPPEFRNE SSLASTLKTL LFFTALMITV PIGLYFTTKS, corresponding to N terminal amino acids 1-50 of Human VMA21

  • Positive control
    • HepG2 cell lysate Human Kidney Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab62591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • RelevanceVMA21 is the homolog of S. cerevisiae's vacuolar H+-ATPase and is essential for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.
  • Cellular localizationEndoplasmic reticulum
  • Database links
  • Alternative names
    • MEAX antibody
    • Myopathy with excessive autophagy protein antibody
    • Vacuolar ATPase assembly integral membrane protein VMA21 antibody
    • VMA21 antibody
    • XMEA antibody
    see all

Anti-VMA21 antibody images

  • All lanes : Anti-VMA21 antibody (ab62591) at 5 µg/ml

    Lane 1 : Molecular Weight Marker
    Lane 2 : HepG2 Cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
    Additional bands at : 30 kDa (possible cross reactivity).
    Antibodies diluted in 5% skim milk / PBS buffer. 15% Gel used.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling VMA21 with ab62591 at 4-8µg/ml. Arrows indicate positively labelled epitehlial cells of the renal tubule. Magnification: 400X.

References for Anti-VMA21 antibody (ab62591)

ab62591 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab62591.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"