Overview

  • Product name
    Anti-VMA21 antibody
  • Description
    Rabbit polyclonal to VMA21
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
  • Immunogen

    A region within synthetic peptide: MERPDKAALN ALQPPEFRNE SSLASTLKTL LFFTALMITV PIGLYFTTKS, corresponding to N terminal amino acids 1-50 of Human VMA21

  • Positive control
    • HepG2 cell lysate Human Kidney Tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab62591 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1:1562500.

IHC-P Use a concentration of 4 - 8 µg/ml.

Target

  • Relevance
    VMA21 is the homolog of S. cerevisiae's vacuolar H+-ATPase and is essential for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.
  • Cellular localization
    Endoplasmic reticulum
  • Database links
  • Alternative names
    • MEAX antibody
    • Myopathy with excessive autophagy protein antibody
    • Vacuolar ATPase assembly integral membrane protein VMA21 antibody
    • VMA21 antibody
    • XMEA antibody
    see all

Images

  • All lanes : Anti-VMA21 antibody (ab62591) at 5 µg/ml

    Lane 1 : Molecular Weight Marker
    Lane 2 : HepG2 Cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 11 kDa
    Observed band size : 11 kDa
    Additional bands at : 30 kDa (possible cross reactivity).Antibodies diluted in 5% skim milk / PBS buffer. 15% Gel used.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling VMA21 with ab62591 at 4-8µg/ml. Arrows indicate positively labelled epitehlial cells of the renal tubule. Magnification: 400X.

References

ab62591 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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