Recombinant Anti-VMAT1 antibody [EPR6476] (ab168347)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6476] to VMAT1
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-VMAT1 antibody [EPR6476]
See all VMAT1 primary antibodies -
Description
Rabbit monoclonal [EPR6476] to VMAT1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human VMAT1. The exact sequence is proprietary.
Database link: P54219 -
Positive control
- BxPC-3, PC-12 and A549 cell lysates, A549 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6476 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
|
Notes |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 56 kDa. |
Target
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Function
Involved in the vesicular transport of biogenic amines. -
Sequence similarities
Belongs to the major facilitator superfamily. Vesicular transporter family. -
Cellular localization
Cytoplasmic vesicle membrane. - Information by UniProt
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Database links
- Entrez Gene: 6570 Human
- Entrez Gene: 110877 Mouse
- Entrez Gene: 25693 Rat
- Omim: 193002 Human
- SwissProt: P54219 Human
- SwissProt: Q8R090 Mouse
- SwissProt: Q01818 Rat
- Unigene: 158322 Human
see all -
Alternative names
- 4832416I10Rik antibody
- Adrenal chromaffin granule amine transporter; CGAT antibody
- CGAT antibody
see all
Images
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Intracellular flow cytometrical analysis of permeabilized A549 cells with ab168347 antibody at a dilution of 1/100 (red) or a rabbit IgG negative (green).
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All lanes : Anti-VMAT1 antibody [EPR6476] (ab168347) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab168347 has been referenced in 3 publications.
- Wu Q et al. Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells. J Neurosci 39:199-211 (2019). PubMed: 30381405
- Sato DX et al. Human-specific mutations in VMAT1 confer functional changes and multi-directional evolution in the regulation of monoamine circuits. BMC Evol Biol 19:220 (2019). PubMed: 31791232
- Zhang Q et al. Differential Co-release of Two Neurotransmitters from a Vesicle Fusion Pore in Mammalian Adrenal Chromaffin Cells. Neuron 102:173-183.e4 (2019). PubMed: 30773347