Anti-Von Willebrand Factor antibody (ab11713)

Overview

  • Product nameAnti-Von Willebrand Factor antibody
    See all Von Willebrand Factor primary antibodies
  • Description
    Sheep polyclonal to Von Willebrand Factor
  • Specificityab11713 detects Human von Willebrand factor.
  • Tested applicationsSuitable for: IHC-Fr, Immunodiffusion, Flow Cyt, ELISA, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Purified human von Willebrand factor.

  • Positive control
    • Tonsil.
  • General notesThis product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.
Immunodiffusion 1/1.
Flow Cyt Use at an assay dependent concentration.

ab37385 - Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionImportant in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
    • Tissue specificityPlasma.
    • Involvement in diseaseDefects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
    • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.
    • DomainThe von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
    • Post-translational
      modifications
      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.
    • Cellular localizationSecreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Coagulation factor VIII antibody
      • Coagulation factor VIII VWF antibody
      • F8VWF antibody
      • Factor VIII related antigen antibody
      • von Willebrand antigen 2 antibody
      • von Willebrand antigen II antibody
      • Von Willebrand disease antibody
      • VWD antibody
      • vWF antibody
      • VWF_HUMAN antibody
      see all

    Anti-Von Willebrand Factor antibody images

    • ab11713 at a 1/1000 dilution staining Von Willebrand Factor in mouse blood vessels by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 1 hour at 19°C. Secondary used at 1/200 polyclonal Donkey anti-sheep conjugated to Alexa Fluor 647 (pink). Counterstain (blue) DAPI.

      See Abreview

    • ab11713 staining Von Willebrand Factor in porcine heart tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in acetone, permeabilized using 0.3% Triton/PBS, blocked with 10% serum for 30 minutes at 22°C and then incubated with ab11713 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated donkey anti-sheep polyclonal used at a 1/1000 dilution.

      See Abreview

    References for Anti-Von Willebrand Factor antibody (ab11713)

    This product has been referenced in:
    • Nurnberg ST  et al. Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap. PLoS Genet 11:e1005155 (2015). Mouse . Read more (PubMed: 26020946) »
    • Cheung K  et al. CD31 signals confer immune privilege to the vascular endothelium. Proc Natl Acad Sci U S A 112:E5815-24 (2015). IHC . Read more (PubMed: 26392551) »

    See all 9 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (A7r5 smooth muscle cells)
    Permeabilization Yes - Triton and Tween
    Specification A7r5 smooth muscle cells
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative Formaldehyde
    Username

    Julie Kohn

    Verified customer

    Submitted May 02 2016

    Application Immunocytochemistry
    Sample Guinea pig Cell (Endothelilal cells freshly isolated)
    Permeabilization Yes - 0.1% Triton X-100
    Specification Endothelilal cells freshly isolated
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 23 2015

    Application Western blot
    Sample Mouse Tissue lysate - whole (Skin)
    Gel Running Conditions Reduced Denaturing (7.5%gel)
    Loading amount 125 µg
    Treatment Wound
    Specification Skin
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 27 2015

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Cell lysate - whole cell (brain, liver)
    Specification brain, liver
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 05 2015

    Application Immunohistochemistry (Frozen sections)
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample Mouse Tissue sections (skin dermis)
    Specification skin dermis
    Permeabilization No
    Fixative Acetone
    Username

    Abcam user community

    Verified customer

    Submitted May 30 2014

    Application Flow Cytometry
    Fixation Paraformaldehyde
    Permeabilization Yes - 0.2% saponin
    Sample Mouse Cell (skin)
    Specification skin
    Preparation Cell harvesting/tissue preparation method: Wilson L, Fathke C, Isik F. Tissue dispersion and flow cytometry for the cellular analysis of wound healing. BioTechniques 2002: 32: 548-551.
    Sample buffer: 2%BSA/PBS
    Username

    Abcam user community

    Verified customer

    Submitted May 14 2014

    Thank you for contacting Abcam.

    I ran an alignment of the human and chicken VWF protein sequence, and there is only 56.1% homology between the two. Our AbTrial program, which allows researchers to test our products in untested species and rece...

    Read More

    Thank you very much for your call today and for your patience while I've been in touch with the production lab regarding your enquiry.
    The IgG concentration of this lot is 9.13 mg/ml.
    I hope that this information will be useful, but if you ha...

    Read More

    Hello! The general protocol for Flow Cytometry is below. The reference for the paper is:
    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2195953/
    Please let me know if you have any further questions!
    Cells Flow cytometry intracellular staini...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Lung)
    Specification Lung
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization No
    Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
    Username

    Mr. Carl Hobbs

    Verified customer

    Submitted Sep 07 2012

    1-10 of 23 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"