Overview

  • Product name
    Anti-Von Willebrand Factor antibody
    See all Von Willebrand Factor primary antibodies
  • Description
    Sheep polyclonal to Von Willebrand Factor
  • Specificity
    ab11713 detects Human von Willebrand factor.
  • Tested applications
    Suitable for: IHC-Fr, Immunodiffusion, Flow Cyt, ELISA, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human, Pig
  • Immunogen

    Purified human von Willebrand factor.

  • Positive control
    • Tonsil.
  • General notes
    This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50 - 1/100.
Immunodiffusion 1/1.
Flow Cyt Use at an assay dependent concentration.

ab37385 - Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
    • Tissue specificity
      Plasma.
    • Involvement in disease
      Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
    • Sequence similarities
      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.
    • Domain
      The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
    • Post-translational
      modifications
      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.
    • Cellular localization
      Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Coagulation factor VIII antibody
      • Coagulation factor VIII VWF antibody
      • F8VWF antibody
      • Factor VIII related antigen antibody
      • von Willebrand antigen 2 antibody
      • von Willebrand antigen II antibody
      • Von Willebrand disease antibody
      • VWD antibody
      • vWF antibody
      • VWF_HUMAN antibody
      see all

    Anti-Von Willebrand Factor antibody images

    • ab11713 at a 1/1000 dilution staining Von Willebrand Factor in mouse blood vessels by Immunohistochemistry (frozen sections) incubated for 16 hours at 4°C. PFA fixed. Permeabilized with 0.1% Triton. Blocked using 20% serum for 1 hour at 19°C. Secondary used at 1/200 polyclonal Donkey anti-sheep conjugated to Alexa Fluor 647 (pink). Counterstain (blue) DAPI.

      See Abreview

    • ab11713 staining Von Willebrand Factor in porcine heart tissue by Immunohistochemistry (Frozen sections).
      Tissue was fixed in acetone, permeabilized using 0.3% Triton/PBS, blocked with 10% serum for 30 minutes at 22°C and then incubated with ab11713 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a FITC conjugated donkey anti-sheep polyclonal used at a 1/1000 dilution.

      See Abreview

    References for Anti-Von Willebrand Factor antibody (ab11713)

    This product has been referenced in:
    • Nurnberg ST  et al. Coronary Artery Disease Associated Transcription Factor TCF21 Regulates Smooth Muscle Precursor Cells That Contribute to the Fibrous Cap. PLoS Genet 11:e1005155 (2015). Mouse . Read more (PubMed: 26020946) »
    • Cheung K  et al. CD31 signals confer immune privilege to the vascular endothelium. Proc Natl Acad Sci U S A 112:E5815-24 (2015). IHC . Read more (PubMed: 26392551) »

    See all 12 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Keloid)
    Permeabilization
    No
    Specification
    Keloid
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted Sep 15 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Lung)
    Permeabilization
    Yes - Triton 0.1%
    Specification
    Lung
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 37°C
    Fixative
    Zinc fixative
    Username

    Abcam user community

    Verified customer

    Submitted Jul 25 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (A7r5 smooth muscle cells)
    Permeabilization
    Yes - Triton and Tween
    Specification
    A7r5 smooth muscle cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde
    Username

    Julie Kohn

    Verified customer

    Submitted May 02 2016

    Application
    Immunocytochemistry
    Sample
    Guinea pig Cell (Endothelilal cells freshly isolated)
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    Endothelilal cells freshly isolated
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Sep 23 2015

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Skin)
    Gel Running Conditions
    Reduced Denaturing (7.5%gel)
    Loading amount
    125 µg
    Treatment
    Wound
    Specification
    Skin
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 27 2015

    Application
    Western blot
    Loading amount
    25 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Mouse Cell lysate - whole cell (brain, liver)
    Specification
    brain, liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 05 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (skin dermis)
    Specification
    skin dermis
    Permeabilization
    No
    Fixative
    Acetone
    Username

    Abcam user community

    Verified customer

    Submitted May 30 2014

    Application
    Flow Cytometry
    Fixation
    Paraformaldehyde
    Permeabilization
    Yes - 0.2% saponin
    Sample
    Mouse Cell (skin)
    Specification
    skin
    Preparation
    Cell harvesting/tissue preparation method: Wilson L, Fathke C, Isik F. Tissue dispersion and flow cytometry for the cellular analysis of wound healing. BioTechniques 2002: 32: 548-551.
    Sample buffer: 2%BSA/PBS
    Username

    Abcam user community

    Verified customer

    Submitted May 14 2014

    Thank you for contacting Abcam.

    I ran an alignment of the human and chicken VWF protein sequence, and there is only 56.1% homology between the two. Our AbTrial program, which allows researchers to test our products in untested species and rece...

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    Thank you very much for your call today and for your patience while I've been in touch with the production lab regarding your enquiry.
    The IgG concentration of this lot is 9.13 mg/ml.
    I hope that this information will be useful, but if you ha...

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