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Human Von Willebrand Factor Antigen prepared from citrated human plasma. Greater than 95% purity by SDS-PAGE.
Our Abpromise guarantee covers the use of ab8820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Double Immunodiffusion||Use at an assay dependent dilution. 10µL antiserum vs 5µL vWF (end dilution 1mg/mL) The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Results can be enhanced by washing completed agarose gels in saline, drying and finally staining with a protein stain such as coomassie brilliant blue or acid blue stain.|
|Counter Current Immunoelectrophoresis||Use at an assay dependent dilution. 20µL antiserum vs 10µL fresh plasma, wells 6mm apart, rim to rim.100v for 30 minutes|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"