Anti-Von Willebrand Factor antibody (ab9378)

Overview

  • Product nameAnti-Von Willebrand Factor antibody
    See all Von Willebrand Factor primary antibodies
  • Description
    Rabbit polyclonal to Von Willebrand Factor
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Factor VIII related antigen isolated from human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab9378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
Flow Cyt 1/250. (see Abreview).



ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 309 kDa).

Target

  • FunctionImportant in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • DomainThe von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • Post-translational
    modifications
    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • Cellular localizationSecreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

Anti-Von Willebrand Factor antibody images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Von Willebrand Factor with ab9378.
  • Anti-Von Willebrand Factor antibody (ab9378) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 309 kDa
    Observed band size : 250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 40 kDa,50 kDa,65 kDa. We are unsure as to the identity of these extra bands.
    Many bands of varying sizes can be seen on Western blots, perhaps indicating differential processing by ADAMTS13 and other enzymes
  • Central Panel: ab9378 staining Von Willebrand Factor in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 568-conjugated Goat polyclonal to rabbit IgG, dilution 1/100, was used as secondary antibody.
    Top panel: Nuclei counterstained with DAPI (blue).
    Bottom panel: Overlay

    See Abreview

  • ab9378 staining Von Willebrand Factor in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    P : Permeabilized;
    US : Unstained, Red Peak;
    IGG RB : IgG Rabbit (Isotype Control), Blue Peak;
    VWF : Von Willebrand Factor antibody, Green peak.

    See Abreview

  • Human normal colon. Staining is localised to storage granules. Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control. Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for rabbit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-Von Willebrand Factor antibody (ab9378)

ab9378 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (frozen bone)
Antigen retrieval step None
Permeabilization Yes - permeabilization
Specification frozen bone
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jul 14 2015

Application Western blot
Sample Human Tissue lysate - whole (liver)
Gel Running Conditions Reduced Denaturing
Loading amount 20 µg
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Jul 13 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample Rat Tissue sections (sciatic nerve)
Specification sciatic nerve
Permeabilization Yes - 0.3% TritonX-100
Fixative Paraformaldehyde
Username

Ms. Hye yeong Lee

Verified customer

Submitted Mar 16 2015

We have antibodies against CD34 and CD105 that are known to be reactive with the human CD antigens, but non-reactiivity with mouse CD antigens is more difficult to demonstrate consistently. We do not have any antibodies against these proteins (or other...

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Thank you for contacting us. All 3 antibodies were raised against the human full length protein. I checked the homology with the mouse full length protein and it is 83%. Cross reactivity might therefore be likely, but has not been tested. Since we do n...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Platelets)
Loading amount 20 µg
Specification Platelets
Treatment ADP for 30 min
Gel Running Conditions Reduced Denaturing (8-16% Biorad Criterion Gel with 18 wells)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 12 2011

Thank you for your enquiry. I have been informed that ab9378 has not been tested for potential reactivity with Factor VIII protein. I hope this is helpful. Please contact me again if you have any further questions.

As detailed the immunogen is the full length human native protein, swiss-prot ID P04275. P04275 Please do not hesitate to contact me should you have further questions.

Thank you for contacting us. The human Von Willebrand Factor has 83% identity with mouse. Both products have been generated from the full length protein. ab6994 Immunogen; Full length native protein (purified from plasma) (Human). Histo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (Platelets)
Specification Platelets
Preparation Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation Paraformaldehyde
Permeabilization Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy Platelets
Username

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 17 2010

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