Anti-Von Willebrand Factor antibody (ab96340)

Overview

  • Product nameAnti-Von Willebrand Factor antibody
    See all Von Willebrand Factor primary antibodies
  • Description
    Rabbit polyclonal to Von Willebrand Factor
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1100 - 1200 of Human Von Willebrand Factor.

  • Positive control
    • This antibody gave a positive signal in Human spleen tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab96340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 275 kDa (predicted molecular weight: 309 kDa).

Target

  • FunctionImportant in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • DomainThe von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • Post-translational
    modifications
    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • Cellular localizationSecreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

Anti-Von Willebrand Factor antibody images

  • Anti-Von Willebrand Factor antibody (ab96340) at 1 µg/ml + Human spleen tissue lysate - total protein (ab29699) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 309 kDa
    Observed band size : 275 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 31 kDa,40 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References for Anti-Von Willebrand Factor antibody (ab96340)

ab96340 has not yet been referenced specifically in any publications.

Product Wall

The following antibodies could be suitable as per your request;

http://www.abcam.com/von-willebrand-factor-antibody-ab96340.html

http://www.abcam.com/von-willebrand-factor-antibody-aminoterminal-end-ab47129.html

Thank you for enquiry.

I am sorry to confirm that asfar as we are aware, ab96340 has never been tested in IHC-P. All tested applications are specified on our datasheets, and these are updated as soon as any new information is brought to our ...

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Vielen Dank für Ihren Anruf.

Nachdem ich wie versprochen alle unsere Von Willebrand Faktor- Antikörperdaraufhin untersucht habe, ob sie möglicherweise die Bindung von Faktor VIII inhibieren können, weiß ich nicht, ob...

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As far as I can see ab96340 should not cross react with Factor VIII. I blasted the immunogen sequence for ab96430 and only vWF came back. I also aligned the immunogen sequence to human factor VIII and there was no significant similarity.

If t...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"