Von Willebrand Factor (VWF) Human ELISA Kit (ab108918)

Overview

  • Product nameVon Willebrand Factor (VWF) Human ELISA KitSee all Von Willebrand Factor kits ...
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    = 2.5 mU/ml
  • Range
    2.5 mU/ml - 80 mU/ml
  • Recovery

    99 %

  • Assay time
    5h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Von Willebrand Factor (VWF) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human Von Willebrand Factor in plasma, serum, and cell culture supernatants.

    A Von Willebrand Factor specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Von Willebrand Factor specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Von Willebrand Factor captured in plate.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionImportant in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • Tissue specificityPlasma.
  • Involvement in diseaseDefects in VWF are the cause of von Willebrand disease type 1 (VWD1) [MIM:193400]. A common hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 1 is characterized by partial quantitative deficiency of circulating von Willebrand factor, that is otherwise structurally and functionally normal. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma.
    Defects in VWF are the cause of von Willebrand disease type 2 (VWD2) [MIM:613554]. A hemorrhagic disorder due to defects in von Willebrand factor protein and resulting in impaired platelet aggregation. Von Willebrand disease type 2 is characterized by qualitative deficiency and functional anomalies of von Willebrand factor. It is divided in different subtypes including 2A, 2B, 2M and 2N (Normandy variant). The mutant VWF protein in types 2A, 2B and 2M are defective in their platelet-dependent function, whereas the mutant protein in type 2N is defective in its ability to bind factor VIII. Clinical manifestations are mucocutaneous bleeding, such as epistaxis and menorrhagia, and prolonged bleeding after surgery or trauma.
    Defects in VWF are the cause of von Willebrand disease type 3 (VWD3) [MIM:277480]. A severe hemorrhagic disorder due to a total or near total absence of von Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. Bleeding usually starts in infancy and can include epistaxis, recurrent mucocutaneous bleeding, excessive bleeding after minor trauma, and hemarthroses.
  • Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • DomainThe von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • Post-translational
    modifications
    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • Cellular localizationSecreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Target information above from: UniProt accession P04275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • Coagulation factor VIII
    • coagulation factor VIII VWF
    • F8VWF
    • F8VWF
    • Factor VIII related antigen
    • FACTOR VIII-VON WILLEBRAND FACTOR
    • von Willebrand antigen 2
    • von Willebrand antigen 2
    • Von Willebrand antigen II
    • von Willebrand antigen II
    • Von Willebrand disease
    • von Willebrand factor
    • VWD
    • VWD
    • VWF
    • vWF
    • VWF_HUMAN
    • VWF_HUMAN
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab108918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent dilution.

Von Willebrand Factor (VWF) Human ELISA Kit images

  • Representative Standard Curve using ab108918

Protocols

References for Von Willebrand Factor (VWF) Human ELISA Kit (ab108918)

ab108918 has not yet been referenced specifically in any publications.

Product Wall

I can confirm that the ELISA kits ab108918 and ab168548 are identical apart from ab168548 has a positive and negative plasma control included. The antibodies used in both of these kits are indeed the same.

Unfortunately I will not be able to send you a sample kit for testing purpose because this is against the company guidelines. All our product are vigorously tested in compatible samples and are fully guaranteed so if journal has any problem we can prov...

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Thank you for contacting us and making us aware of the typo in our protocol.

The corrected text on pg.8 of protocol last sentence of 3. Standard Curve which used to read: Diluent serves as the zero standard (0 µg/ml), now reads: “Diluent serv...

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Merci beaucoup pour votre émail et votre retour. Nous sommes très heureux de savoir que le problème s'est donc expliqué et résolu.

J'espère que vous avez bien reçu le kit complémentaire ...

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Merci de votre patience.

Voici les réponses à vos questions:

Le laboratoire à confirmé que c'est normal de voir des cristaux aussi dans le tampon du diluant. S'il y à des cristaux dans le tampon, ...

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Merci beaucoup pour votre émail.

Nous allons vous envoyer le kit lundi, vous devriez le recevoir donc mardi de la semaine prochaine. Veuillez s'il vous plaît nous tenir aussi au courant, sile problème est complèteme...

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Merci pour votre émail et votre patience.

Je peux vous confirmer qu'il s'agit d'une erreur d'annotation. Effectivement, les standards que vous avez reçu dans le kit lot GR79395 ne sont pas à 50mU, mais à 160mU. Read More

Merci beaucoup pour votre émail.
Je suis très désolée de ce problème que vous avez rencontré avec le standard du nouveau lot. Je peux vous assurer que nous prenons ceci très aux sérieux. Je suis en train d'investiguer ce problème et j'ai contacté ...

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Thank you for your inquiry.

The kit use purified vWF from plasma as standard. The standard has some factor VIII, but not a significant amount.

Please note that when you receive this kit, the concentration of the standard will be o...

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Les unités utilisés dans ce kit sont par rapport a une unité de référence interne, qui avait été utilisé comme contrôle. Pour l'instant, cet unité n'as pas été quantifié pour le contenue de vWF, mais le laboratoire planifie de faire la conversion ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"